Meckelin Rabbit Polyclonal Antibody
Catalog# 0903-7
Meckelin Rabbit Polyclonal Antibody
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WB
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IHC-P
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FC
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Human
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Mouse
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unconjugated
Overview
Product Name
Meckelin Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human Meckelin aa 20-80.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P, FC
Target Molecular Weight
112 kDa
Positive Control
MCF7 cell lysates, Hela cell lysate, mouse kidney tissue, human placenta tissue, human breast cancer tissue, PANC-1.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
Target
Function
Required for ciliary structure and function. Part of the tectonic-like complex which is required for tissue-specific ciliogenesis and may regulate ciliary membrane composition (By similarity). Involved in centrosome migration to the apical cell surface during early ciliogenesis. Involved in the regulation of cilia length and appropriate number through the control of centrosome duplication. Required for cell branching morphology. Essential for endoplasmic reticulum-associated degradation (ERAD) of surfactant protein C (SFTPC).
Background References
1. Dawe H.R.et.al.The Meckel-Gruber syndrome proteins MKS1 and meckelin interact and are required for primary cilium formation.Hum. Mol. Genet. 16:173-186(2007).
Subcellular Location
Cytoskeleton, Plasma membrane, Endoplasmic reticulum.
Synonyms
JBTS6 antibody
Meckel syndrome type 3 protein antibody
MKS3 antibody
NPHP11 antibody
TMEM67 antibody
TNEM67 antibody
Transmembrane protein 67 antibody
Images
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Western blot analysis of Meckelin on different lysates with Rabbit anti-Meckelin antibody (0903-7) at 1/1,000 dilution.
Lane 1: MCF7 (Human breast cancer cell) cell lysate
Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 110s seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: 0903-7, 1/5,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 112 kDa
Observed band size: 100 kDa -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Meckelin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0903-7, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Meckelin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0903-7, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Meckelin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0903-7, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Flow cytometric analysis of Meckelin was done on PANC-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (0903-7, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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