Rabbit Monoclonal Antibody (mAb) Service
HUABIO offers a comprehensive range of high-quality custom rabbit monoclonal antibody production services to meet your research needs. Since 2007, our team has successfully produced over 10,000 custom antibodies from rabbit, mouse, rat, and various plant hosts. We provide industry-leading guarantees to ensure that the antibodies can recognize the target protein in WB experiments. Simply provide the target sequence, and HUABIO will begin the antibody production process to help you achieve your research goals.
Highlights
Flexible Antigen Formats
From proteins to peptides, cells, and nucleic acids — we accommodate diverse antigens to tackle even the most challenging targets.
High-Throughput Single B Cell Screening
Efficiently identify top-performing clones with our powerful single B cell screening platform — fast, precise, and reliable.
Fast Turnaround – Just 2 Weeks
Go from cell sorting to supernatant in as little as 2 weeks. Speed up your research without compromising quality.
Comprehensive Validation, Ready to Use
Each antibody is rigorously tested across ELISA, WB, FC, and IF — ensuring high performance and immediate usability.
Rabbit Monoclonal Antibody (mAb) Service Details
| Stage | Details | Time | Deliverables | QC Standard |
|---|---|---|---|---|
| Immunogen Preparation |
1. Conventional peptide projects (13-18aa, purity >85%) 2. Modified peptide project A (monophosphorylation/monoacetylation) 3. Modified peptide project B (dual phosphorylation/lactylation/crotonylation/succinylation/methylation) 4. Prokaryotic protein project C (<1000bp gene fragment synthesis + protein expression and purification, purity >85%) 5. Prokaryotic protein project D (customer provides expression plasmid, only protein expression and purification is performed, purity >85%) 6. Eukaryotic protein project E (<1500bp gene fragment synthesis + protein expression and purification, purity >85%) 7. Eukaryotic protein project F (1500-2500bp gene fragment synthesis + protein expression and purification, purity >85%) |
3-8 weeks (depends on immunogen types) | Peptide report, Protein report | Mass Spectrometry Report for peptides, SDS-PAGE result for proteins |
| Rabbit Immunization and Serum Titer Test |
2 rabbits, 7mg immunogens at least (>85% purity, >0.5mg/ml), serum titer standard 1:64000 range from 0.2-0.6; For more animal immunization: adding one more rabbit, and 3mg more immunogen are required. |
8-12 weeks | Antiserum report, Small scale pAb purification (optional) | Indirect ELISA |
| 1st Round Single B Cell Sorting | One 96-well plate (94 cells, the remaining wells for positive control), at least 10 ELISA positive clones (OD450>0.5, 1:10 & 1:100 supernatant dilution for indirect ELISA assay, different antibody VH/VL sequences) with about 1.5ml antibody supernatant. | 1 week | Sorting record and report | Indirect ELISA of diluted supernatants |
| Antibody Production and Screening | Production of antibody and screening. | 1 week | Indirect ELISA data/Immunogen-positive clones report, 1.5ml antibody supernatant delivery (at least 10 clones) | Indirect ELISA |
| Antibody Sequencing | All ELISA positive clones are sequenced. Up to 94 clones can be sequenced in one round. | 2 weeks | Sequencing and alignment | / |
| Clone Picking | According to the client's experiment, 2 clones will be picked for antibody production. | / | (Additional clone picked for production options available) | / |
| Antibody Production and ELISA Validation |
Indirect ELISA assay to check the binding of immunogen and antibodies. 1-1.5mg antibodies will be provided. (Usually antibodies VH/VL sequences are provided but not plasmids. ) |
4 weeks |
Antibody sequence, VH/VL plasmids, purified antibody, Indirect ELISA data (Additional clone sequence/purified antibody and exclusive right options available) |
Indirect ELISA |
| Antibody Delivery | Stored in PBS buffer or clients' requested buffer. | / | / | / |