PRODUCT CODE: ET1607-1

CDC7 Kinase Recombinant Rabbit Monoclonal Antibody [SY21-01] (ET1607-1)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of CDC7 Kinase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-1, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Jurkat cell lysate<br />
Lane 2: Hela cell lysate
  • Western blot analysis of CDC7 Kinase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-1, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Jurkat cell lysate<br />
Lane 2: Hela cell lysate
  • Flow cytometric analysis of CDC7 Kinase was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1607-1, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of CDC7 Kinase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-1, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Jurkat cell lysate
Lane 2: Hela cell lysate

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

CDC7 Kinase Recombinant Rabbit Monoclonal Antibody [SY21-01] (ET1607-1)

Immunogen

Synthetic peptide within human cdc7 kinase aa 1-50 / 574.

Host

Rabbit

Positive Control

Jurkat, A431, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SY21-01

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

64 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

CDC7 Kinase

SYNONYMS

Cdc 7 antibody; CDC7 antibody; CDC7 cell division cycle 7 antibody; CDC7 cell division cycle 7 like 1 antibody; CDC7 L1 antibody; Cdc7 like 1 antibody; CDC7 related kinase antibody; CDC7-related kinase antibody; CDC7_HUMAN antibody; CDC7L 1 antibody; CDC7L1 antibody; Cell division cycle 7 homolog antibody; Cell division cycle 7 kinase antibody; Cell division cycle 7 like protein 1 antibody; Cell division cycle 7 related protein kinase antibody; Cell division cycle 7-related protein kinase antibody; HsCDC 7 antibody; HsCdc7 antibody; Hsk 1 antibody; Hsk1 antibody; HuCDC 7 antibody; huCdc7 antibody; MGC117361 antibody; MGC126237 antibody; MGC126238 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC7 subfamily.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

The Dbf4/Cdc7 protein kinase is essential for the activation of replication origins during S phase. Cdc7/Dbf4 efficiently phosphorylates several proteins that are required for the initiation of DNA replication, including five of the six minichromosome maintenance (Mcm) proteins and the p180 subunit of DNA polymerase a-primase. This protein complex consists of the catalytic subunit Cdc7 associating with the regulatory and activating subunit Dbf4. The kinase activity of the complex is regulated throughout the cell cycle, mainly by fluctuating levels of Dbf4. Cdc7 is consistently expressed throughout the cell cycle, while the expression of Dbf4 is absent during G1 phase and accumulates during S and G2 phases. The anaphase-promoting complex rapidly degrades Dbf4 at the time of chromosome segregation, and the stability of Dbf4 remains low during pre-Start G1 phase. The coordinated degradation of Dbf4 and the time of chromosome separation is important to ensuring that prereplicative complexes, which assemble after chromosome segregation, do not immediately refire.