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Mouse CCL3/MIP-1 alpha enzyme-linked immunoassay kit (EM0031)

Price: Regular price $499.00

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Catalog# EM0031

Assay type Sandwich ELISA

Standard curve range 1.37~1000 pg/ml

Sensitivity 0.51 pg/ml (10 μl)

Reacts with

  • Mouse

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01590.01410.0150
1.370.02320.02100.02210.0071
4.120.03410.03020.03220.0172
12.350.06510.06580.06550.0505
37.040.17030.17070.17050.1555
111.110.45070.43180.44130.4263
333.331.27301.22001.24651.2315
1000.003.18703.28003.23353.2185

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)18.888.6247.518.596.2282.2
Standard Deviation1.43.913.21.26.710.1
Coefficient of Variation(%)7.34.45.36.66.93.6

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum301.2-20.81004.19

Sensitivity

0.51 pg/ml (10 μl)

Standard curve range

1.37~1000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of mouse CCL3 into health mouse serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 106% to 139% with an overall mean recovery of 128%.

Specifications

Product Type

ELISA Kit

Product Name

Mouse CCL3/MIP-1 alpha enzyme-linked immunoassay kit (EM0031)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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