The Nanobody Foundation
With the emergence of recombinant DNA technology, many antibody fragments have been developed devoid of undesired properties of natural immunoglobulins. Among them, camelid heavy-chain variable domains or nanobodies. By definition, nanobodies are single-domain antibodies derived from the variable regions of Camelidae (e.g., camels and llamas) atypical immunoglobulins. Camelidae possess fully functional antibodies that consist of only heavy chains. These single variable domains have full antigen binding capacity and, because of their simple structure, are very stable.
Compared to a traditional antibody, nanobodies are 1/10 of the size (~15kDa unconjugated) which means they can penetrate tissue faster and deeper than their antibody counterparts. Since nanobodies are recombinantly produced from clonal DNA, you are guaranteed the same product every time. Comparatively, polyclonal antibodies are produced in live animals and have a very low lot-to-lot consistency while monoclonal antibodies can suffer from drift. From a production point of view, nanobodies are easier to develop and produce in the lab than monoclonal or polyclonal antibodies.
So, What is a Nanoselector?
Our Nanoselector technology is composed of recombinant high-affinity nanobodies covalently coupled to agarose beads. Our cutting-edge nanobodies are camelid antibodies composed only of heavy chains in which the target recognition module is composed of a single variable domain. They are the smallest functional antigen-binding fragment and ideal for rapid, reliable, and specific immunoprecipitation. In short, they’re a fast, reliable and efficient one-step immunoprecipitation assay.
Benefits of using HUABIO Nanoselectors:
- No heavy & light antibody chains
- Low host cell protein background
- Very efficient pulldown, no protein in flow through
- High affinity to bind
- Save time in lab with short incubation
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Find our nanoselector catalog here.