Xanthine Oxidase Mouse Monoclonal Antibody [1C8]
Catalog# EM1701-56
Xanthine Oxidase Mouse Monoclonal Antibody [1C8]
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IHC-P
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FC
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ELISA
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Human
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unconjugated
Overview
Product Name
Xanthine Oxidase Mouse Monoclonal Antibody [1C8]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within Human Xanthine Oxidase aa 1,082-1,131 / 1,333.
Species Reactivity
Human
Validated Applications
IHC-P, FC, ELISA
Molecular Weight
Predicted band size: 146 kDa
Positive Control
Human liver tissue, human kidney tissue, LOVO.
Conjugation
unconjugated
Clone Number
1C8
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
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ELISA
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1:5,000-1:10,000
Target
Function
The process of metabolizing purines to a common molecule known as xanthine is an essential process for the proper shuttling of uric acid. Xanthine oxidase is a flavoprotein enzyme that coordinates molybdenum and utilizes NAD+ as an electron acceptor to catalyze the oxidation of hypoxanthine to xanthine and then to uric acid. The predominant form of this enzyme is xanthine dehydrogenase, which is a homodimer that can be converted to xanthine oxidase by sulfhydryl oxidation or proteolytic modification. Xanthine oxidase is present in species ranging from bacteria to human and is ubiquitously expressed in mammalian tissues. In the oxidase form, this enzyme is coupled to the generation of free radicals. Individuals showing marked elevation of serum xanthine oxidase is suggestive of chronic liver disease and cholestasis, which is a condition defined by hepatic obstruction. Hepatic obstruction causes bile salts, the bile pigment bilirubin, and fats to accumulate in the blood stream instead of being eliminated normally. The clinical consequences of defects in xanthine oxidase range from mild to severe and even contribute to fatal disorders.
Background References
1. Brand S et al. Oxidative DNA damage in kidneys and heart of hypertensive mice is prevented by blocking angiotensin II and aldosterone receptors. PLoS One 9:e115715 (2014).
2. Brem R et al. Oxidation-mediated DNA cross-linking contributes to the toxicity of 6-thioguanine in human cells. Cancer Res 72:4787-95 (2012).
Sequence Similarity
Belongs to the xanthine dehydrogenase family.
Tissue Specificity
Detected in milk (at protein level).
Post-translational Modification
Subject to partial proteolysis; this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).; Contains sulfhydryl groups that are easily oxidized (in vitro); this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).
Subcellular Location
Cytoplasm. Secreted. Peroxisome.
UNIPROT
Synonyms
Xanthine dehydrogenase antibody
Xanthine dehydrogenase/oxidase antibody
Xanthine oxidase antibody
Xanthine oxidoreductase antibody
XD antibody
XDH antibody
XDH_HUMAN antibody
xdha antibody
XO antibody
xor antibody
Images
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Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-xanthine dehydrogenase antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-xanthine dehydrogenase antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"