PRODUCT CODE: ET7107-01

VPS35 Recombinant Rabbit Monoclonal Antibody [JB33-82] (ET7107-01)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of VPS35 on different lysates using anti-VPS35 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: PC-3M<br />
      Lane 2: U937<br />
 Lane 3: SH-SY-5Y
  • Western blot analysis of VPS35 on different lysates using anti-VPS35 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: PC-3M<br />
      Lane 2: U937<br />
 Lane 3: SH-SY-5Y
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-VPS35 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-VPS35 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-VPS35 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-VPS35 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of 293T cells with VPS35 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of VPS35 on different lysates using anti-VPS35 antibody at 1/500 dilution.
Positive control:
Lane 1: PC-3M
Lane 2: U937
Lane 3: SH-SY-5Y

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

VPS35 Recombinant Rabbit Monoclonal Antibody [JB33-82] (ET7107-01)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

PC-3M, U937, SH-SY-5Y, human colon cancer tissue, human kidney tissue, human placenta tissue, mouse colon tissue, 293T.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB33-82

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

91 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:100-1:300

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

VPS35

SYNONYMS

DKFZp434E1211 antibody; DKFZp434P1672 antibody; FLJ10752 antibody; FLJ13588 antibody; FLJ20388 antibody; hVPS35 antibody; Maternal embryonic 3 antibody; Maternal-embryonic 3 antibody; MEM 3 antibody; MEM3 antibody; PARK17 antibody; TCCCTA00141 antibody; Vacuolar protein sorting 35 (yeast) antibody; Vacuolar protein sorting 35 antibody; Vacuolar protein sorting 35 homolog antibody; Vacuolar protein sorting associated protein 35 antibody; Vacuolar protein sorting-associated protein 35 antibody; Vesicle protein sorting 35 antibody; VPS 35 antibody; VPS35 antibody; VPS35_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the VPS35 family.

TISSUE SPECIFICITY

Ubiquitous. Highly expressed in heart, brain, placenta, skeletal muscle, spleen, thymus, testis, ovary, small intestine, kidney and colon.

SUBCELLULAR LOCATION

Cytoplasm. Membrane. Endosome.

FUNCTION

Vacuolar sorting proteins (VPSs) are required for proper trafficking of endocytic and biosynthetic proteins to the vacuole and play an important role in the budding process of cells. VPS35 (vacuolar protein sorting 35), also known as MEM3, is the 796 amino acid human homolog of the S. cerevisiae Vps35 protein. Localized to the cytoplasm and to the peripheral membrane, VPS35 is an essential component of the retromer complex which is involved in retrieval of lysosomal enzyme receptors from endosomes to the trans-Golgi network. VPS35 is expressed ubiquitously with highest expression in heart, placenta, brain, testis, kidney, colon, ovary and spleen. In addition to its crucial role in the retromer complex, VPS35 is part of a subcomplex that is required to regulate transcytosis of the polymeric immunoglobulin receptor from the basolateral to the apical surface of epithelial cells and hepatocytes.