PRODUCT CODE: ER30607

VEGF Antibody (ER30607)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of VEGF on different cell lysates using anti- VEGF antibody at 1/500 dilution.<br />
 Positive control: <br />
 Lane 1: Jurkat <br />
 Lane 2: Hela <br />
 Lane 3: MCF-7 <br />
 Lane 4: Human brain
  • Western blot analysis of VEGF on different cell lysates using anti- VEGF antibody at 1/500 dilution.<br />
 Positive control: <br />
 Lane 1: Jurkat <br />
 Lane 2: Hela <br />
 Lane 3: MCF-7 <br />
 Lane 4: Human brain
  • ICC staining VEGF in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining VEGF in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-VEGF antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with VEGF antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Western blot analysis of VEGF on different cell lysates using anti- VEGF antibody at 1/500 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: Hela
Lane 3: MCF-7
Lane 4: Human brain

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

VEGF Antibody (ER30607)

Immunogen

Synthetic peptide within n terminal of human vegfa.

Host

Rabbit

Positive Control

MCF-7, Hela, Jurkat, HUVEC, human brain tissue, human lung carcinoma tissue, HepG2.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

27 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200

  • FC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

VEGF

SYNONYMS

Folliculostellate cell-derived growth factor antibody; Glioma-derived endothelial cell mitogen antibody; MGC70609 antibody; MVCD1 antibody; Vascular endothelial growth factor A antibody; vascular endothelial growth factor A121 antibody; vascular endothelial growth factor A165 antibody; vascular endothelial growth factor antibody; Vascular permeability factor antibody; VEGF A antibody; Vegf antibody; VEGF-A antibody; VEGF120 antibody; Vegfa antibody; VEGFA_HUMAN antibody; VPF antibody

SEQUENCE SIMILARITIES

Belongs to the PDGF/VEGF growth factor family.

TISSUE SPECIFICITY

Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. A higher level expression seen in pituitary tumors as compared to the pituitary gland.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development.

CITATIONS

  • Tang, Li et al.

    Deficiency of DICER reduces the invasion ability of trophoblasts and impairs the pro-angiogenic effect of trophoblast-derived microvesicles. | Journal of Cellular and Molecular Medicine [2020]

  • Xu, Xiaodong et al.

    Upregulation of miRNA��?01a��?p promotes tumor progression in gastric cancer by suppressing NKRF and activating NF����B signaling. | International Journal of Oncology [2020]