Positive control:
Lane 1: L929
Lane 2: F9
Lane 3: A549
Lane 4: MCF-7
Lane 5: Hela
Lane 6: NIH/3T3
Lane 7: N2A
Lane 8: Jurkat
Lane 9: HepG2
Lane 10: A431
Lane 11: Mouse heart
Lane 12: Mouse brain
Lane 13: Mouse liver
Lane 14: Mouse kidney
Applications
-
WB
-
ICC
-
IHC-P
REACTIVITY
-
Human
-
Mouse
-
Zebrafish
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
VCP Rabbit Polyclonal Antibody (ER30603)
Immunogen
Synthetic peptide within residues of vcp aa 757-806 / 806.
Host
Rabbit
Positive Control
L929, F9, SW480, A549, MCF-7, Hela, NIH/3T3, N2A, Jurkat, HepG2, A431,mouse brain tissue, mouse heart tissue, mouse liver tissue, mouse kidney tissue,human uterus tissue,SHG-44 cells ,mouse skeletal muscle tissue .
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Peptide affinity purified
MOLECULAR WEIGHT
89 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:2,000
-
ICC
-
1:200
-
IHC-P
-
1:200
TARGET
UNIPROT #
PROTEIN NAME
VCP
SYNONYMS
15S Mg(2+) ATPase p97 subunit antibody; 15S Mg(2+)-ATPase p97 subunit antibody; ALS14 antibody; ATPase p97 antibody; CDC48 antibody; IBMPFD antibody; MGC131997 antibody; MGC148092 antibody; MGC8560 antibody; p97 antibody; TER ATPase antibody; TERA antibody; TERA_HUMAN antibody; Transitional endoplasmic reticulum ATPase antibody; Valosin containing protein antibody; Valosin-containing protein antibody; VCP antibody; Yeast Cdc48p homolog antibody
SEQUENCE SIMILARITIES
Belongs to the AAA ATPase family.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated by tyrosine kinases in response to T-cell antigen receptor activation. Phosphorylated in mitotic cells.; ISGylated.; Methylation at Lys-315 catalyzed by VCPKMT is increased in the presence of ASPSCR1. Lys-315 methylation may decrease ATPase activity.
SUBCELLULAR LOCATION
Cytoplasm, nucleus, endoplasmic reticulum
FUNCTION
Valosin-containing protein (VCP) is a highly conserved, that belongs to the AAA (ATPase associated with a variety of cellular activities) family of proteins. It is necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. VCP is involved in the formation of the transitional endoplasmic reticulum (tER). VCP homo-hexamers associate with a variety of protein cofactors to form many distinct protein complexes, which act as chaperones to unfold proteins and transport them to specific cellular compartments or to the proteosome.