PRODUCT CODE: ER30603

VCP Rabbit Polyclonal Antibody (ER30603)

  • Zebrafish

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Zebrafish

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Western blot analysis of VCP on different cell lysates using anti- VCP antibody at 1/2000 dilution.<br />
Positive control:    <br />
Lane 1: L929           <br />
Lane 2: F9  <br />
Lane 3: A549           <br />
Lane 4: MCF-7  <br />
Lane 5: Hela           <br />
Lane 6: NIH/3T3  <br />
Lane 7: N2A            <br />
Lane 8: Jurkat  <br />
Lane 9: HepG2          <br />
Lane 10: A431  <br />
Lane 11: Mouse heart  <br />
 Lane 12: Mouse brain  <br />
Lane 13: Mouse liver   <br />
Lane 14: Mouse kidney
  • Western blot analysis of VCP on different cell lysates using anti- VCP antibody at 1/2000 dilution.<br />
Positive control:    <br />
Lane 1: L929           <br />
Lane 2: F9  <br />
Lane 3: A549           <br />
Lane 4: MCF-7  <br />
Lane 5: Hela           <br />
Lane 6: NIH/3T3  <br />
Lane 7: N2A            <br />
Lane 8: Jurkat  <br />
Lane 9: HepG2          <br />
Lane 10: A431  <br />
Lane 11: Mouse heart  <br />
 Lane 12: Mouse brain  <br />
Lane 13: Mouse liver   <br />
Lane 14: Mouse kidney
  • ICC staining VCP in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining VCP in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining VCP in SHG-44 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining VCP in SW480 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-VCP antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-VCP antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-VCP antibody. Counter stained with hematoxylin.
Western blot analysis of VCP on different cell lysates using anti- VCP antibody at 1/2000 dilution.
Positive control:
Lane 1: L929
Lane 2: F9
Lane 3: A549
Lane 4: MCF-7
Lane 5: Hela
Lane 6: NIH/3T3
Lane 7: N2A
Lane 8: Jurkat
Lane 9: HepG2
Lane 10: A431
Lane 11: Mouse heart
Lane 12: Mouse brain
Lane 13: Mouse liver
Lane 14: Mouse kidney

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Zebrafish

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

VCP Rabbit Polyclonal Antibody (ER30603)

Immunogen

Peptide

Host

Rabbit

Positive Control

L929, F9, SW480, A549, MCF-7, Hela, NIH/3T3, N2A, Jurkat, HepG2, A431,mouse brain tissue, mouse heart tissue, mouse liver tissue, mouse kidney tissue,human uterus tissue,SHG-44 cells ,mouse skeletal muscle tissue .

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

89 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:2,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

VCP

SYNONYMS

15S Mg(2+) ATPase p97 subunit antibody; 15S Mg(2+)-ATPase p97 subunit antibody; ALS14 antibody; ATPase p97 antibody; CDC48 antibody; IBMPFD antibody; MGC131997 antibody; MGC148092 antibody; MGC8560 antibody; p97 antibody; TER ATPase antibody; TERA antibody; TERA_HUMAN antibody; Transitional endoplasmic reticulum ATPase antibody; Valosin containing protein antibody; Valosin-containing protein antibody; VCP antibody; Yeast Cdc48p homolog antibody

SEQUENCE SIMILARITIES

Belongs to the AAA ATPase family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by tyrosine kinases in response to T-cell antigen receptor activation. Phosphorylated in mitotic cells.; ISGylated.; Methylation at Lys-315 catalyzed by VCPKMT is increased in the presence of ASPSCR1. Lys-315 methylation may decrease ATPase activity.

SUBCELLULAR LOCATION

Cytoplasm, nucleus, endoplasmic reticulum

FUNCTION

Valosin-containing protein (VCP) is a highly conserved, that belongs to the AAA (ATPase associated with a variety of cellular activities) family of proteins. It is necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. VCP is involved in the formation of the transitional endoplasmic reticulum (tER). VCP homo-hexamers associate with a variety of protein cofactors to form many distinct protein complexes, which act as chaperones to unfold proteins and transport them to specific cellular compartments or to the proteosome.