Positive control:
Lane 1: Hela cell lysate
Lane 2: Human liver tissue lysate
Applications
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WB
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ICC
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IHC-P
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IP
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FC
REACTIVITY
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Human
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Mouse
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Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
TXNRD1 Recombinant Rabbit Monoclonal Antibody [JA11-32] (ET1704-81)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
Human liver tissue lysate, Hela, HUVEC, SH-SY5Y, mouse testes tissue, human kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JA11-32
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
55 kDa
Isotype
IgG
APPLICATION DILUTION
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WB
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1:500-1:2,000
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ICC
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
TXNRD1
SYNONYMS
cytoplasmic antibody; Gene associated with retinoic and IFN-induced mortality 12 protein antibody; Gene associated with retinoic and interferon-induced mortality 12 protein antibody; Gene associated with retinoid IFN induced mortality 12 protein antibody; GRIM 12 antibody; GRIM-12 antibody; GRIM12 antibody; KDRF antibody; KM 102 derived reductase like factor antibody; KM-102-derived reductase-like factor antibody; MGC9145 antibody; Oxidoreductase antibody; Thioredoxin reductase 1 antibody; Thioredoxin reductase 1 cytoplasmic antibody; Thioredoxin reductase GRIM 12 antibody; Thioredoxin reductase TR1 antibody; TR 1 antibody; TR antibody; TR1 antibody; TRXR 1 antibody; TRXR1 antibody; TRXR1_HUMAN antibody; TXNR antibody; TXNRD 1 antibody; Txnrd1 antibody
SEQUENCE SIMILARITIES
Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
TISSUE SPECIFICITY
Isoform 1 is expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver.
POST-TRANSLATIONAL MODIFICATION
The N-terminus of isoform 5 is blocked.; ISGylated.
SUBCELLULAR LOCATION
Cytoplasm. Nucleus.
FUNCTION
Thioredoxin (Trx) is a redox protein that is found in several species, such as bacteria, plants and mammals, and contains a conserved active site, consisting of Trp-Cys-Gly-Pro-Cys. Trx reductases (TrxR1 and TrxR2) are ubiquitous Ly noble flavoproteins that catalyze the NADPH-dependent reduction of Trx as well as several other oxidized cellular components. Mammalian Trx reductases are a part of a selenium-containing pyridine nucleotide-disulphide oxidoreductase family, which has a conserved catalytic site of Cys-Val- Asn-Val-Gly-Cys. TrxR1 and TrxR2 are also involved in the prevention of oxidative stress. Inhibition of TrxR activity may provide for potential treatments of cancer, AIDS and other autoimmune diseases as well as bacterial infections and parasitic diseases.
CITATIONS
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Yu, Jia-Tian et al.
Up-regulation of antioxidative proteins TRX1, TXNL1 and TXNRD1 in the cortex of PTZ kindling seizure model mice. | PloS One [2019]
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WB
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mouse
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Citation
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