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Thioredoxin 2 Recombinant Rabbit Monoclonal Antibody [JE35-76]

Usd: 385

Specification

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Catalog# HA722142

Thioredoxin 2 Recombinant Rabbit Monoclonal Antibody [JE35-76]

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Thioredoxin 2 Recombinant Rabbit Monoclonal Antibody [JE35-76]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Thioredoxin 2 aa 67-166 / 166.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P

Target Molecular Weight

Predicted band size: 18 kDa

Positive Control

Raji cell lysate, K-562 cell lysate, HeLa cell lysate, HepG2 cell lysate, C2C12 cell lysate, C6 cell lysate, PC-12 cell lysate, HeLa, human liver tissue, mouse kidney tissue.

Conjugation

unconjugated

Clone Number

JE35-76

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:100

  • IHC-P

  • 1:2,000

Target

Function

Thioredoxin, mitochondrial also known as thioredoxin-2 is a protein that in humans is encoded by the TXN2 gene on chromosome 22. This nuclear gene encodes a mitochondrial member of the thioredoxin family, a group of small multifunctional redox-active proteins. The encoded protein may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis.

Background References

1. Huang Y et al. Brown adipose TRX2 deficiency activates mtDNA-NLRP3 to impair thermogenesis and protect against diet-induced insulin resistance. J Clin Invest. 2022 May

2. Wang X et al. Thioredoxin-2 impacts the inflammatory response via suppression of NF-κB and MAPK signaling in sepsis shock. Biochem Biophys Res Commun. 2020 Apr

Subcellular Location

Mitochondrion.

UNIPROT

SwissProt: Q99757 Human

SwissProt: P97493 Mouse

SwissProt: P97615 Rat

Synonyms

mitochondrial thioredoxin antibody

MT-TRX antibody

MTRX antibody

thioredoxin 2 precursor antibody

Thioredoxin mitochondrial antibody

Thioredoxin2 antibody

TRX 2 antibody

TRX2 antibody

TXN 2 antibody

TXN2 antibody

Images

  • Western blot analysis of Thioredoxin 2 on different lysates with Rabbit anti-Thioredoxin 2 antibody (<a href="/products/HA722142" style="font-weight: bold;text-decoration: underline;">HA722142</a>) at 1/1,000 dilution.<br /><br />Lane 1: Raji cell lysate<br />Lane 2: K-562 cell lysate<br />Lane 3: HeLa cell lysate<br />Lane 4: HepG2 cell lysate<br />Lane 5: C2C12 cell lysate<br />Lane 6: C6 cell lysate<br />Lane 7: PC-12 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 18 kDa<br />Observed band size: 12 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722142" style="font-weight: bold;text-decoration: underline;">HA722142</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Thioredoxin 2 on different lysates with Rabbit anti-Thioredoxin 2 antibody (HA722142) at 1/1,000 dilution.

    Lane 1: Raji cell lysate
    Lane 2: K-562 cell lysate
    Lane 3: HeLa cell lysate
    Lane 4: HepG2 cell lysate
    Lane 5: C2C12 cell lysate
    Lane 6: C6 cell lysate
    Lane 7: PC-12 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 18 kDa
    Observed band size: 12 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722142) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of HeLa cells labeling Thioredoxin 2 with Rabbit anti-Thioredoxin 2 antibody (<a href="/products/HA722142" style="font-weight: bold;text-decoration: underline;">HA722142</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Thioredoxin 2 antibody (<a href="/products/HA722142" style="font-weight: bold;text-decoration: underline;">HA722142</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HeLa cells labeling Thioredoxin 2 with Rabbit anti-Thioredoxin 2 antibody (HA722142) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Thioredoxin 2 antibody (HA722142) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Thioredoxin 2 antibody (<a href="/products/HA722142" style="font-weight: bold;text-decoration: underline;">HA722142</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722142" style="font-weight: bold;text-decoration: underline;">HA722142</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Thioredoxin 2 antibody (HA722142) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722142) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Thioredoxin 2 antibody (<a href="/products/HA722142" style="font-weight: bold;text-decoration: underline;">HA722142</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722142" style="font-weight: bold;text-decoration: underline;">HA722142</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Thioredoxin 2 antibody (HA722142) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722142) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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