T7 tag Mouse Monoclonal Antibody [4-C2] (EM50802)
Catalog# EM50802
Mouse Monoclonal to T7 tag
Tested for
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WB
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ELISA
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IP
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IF-Cell
Reacts with
-
Species independent
Western blot analysis of T7 tag on different T7 tagged recombinant proteins with Mouse anti-T7 tag antibody (EM50802) at 1/1,000 dilution.
Lysates/proteins at 50 ng/Lane.
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM50802) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lysates/proteins at 50 ng/Lane.
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM50802) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.