Western blot analysis of T7 tag on T7-tagged recombinant protein using anti-T7 tag antibody at 1/1000 dilution.

Applications

WB
ELISA
IP
ICC

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

T7 tag Mouse Monoclonal Antibody [4-C2] (EM50802)

Immunogen

Peptide

Host

Mouse

Positive Control

T7-tagged recombinant protein.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

4-C2

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

Isotype

IgG1

APPLICATION DILUTION

WB
1:1,000

TARGET

PROTEIN NAME

T7 tag

SYNONYMS

10 antibody; Major capsid protein 10A antibody; Major capsid protein antibody; Minor capsid protein 10B antibody; T7 epitope tag antibody

FUNCTION

The T7 tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag has been used extensively as a general epitope tag in many expression vectors including the pET system that is based on T7 RNA polymerase expression systems. This antibody recognizes the T7-tag fused to either the amino- or carboxy-terminus of targeted proteins.