PRODUCT CODE: ER1901-40

Syntrophin alpha 1 Rabbit Polyclonal Antibody (ER1901-40)

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Syntrophin alpha 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Mouse skeletal muscle tissue lysate<br />
Lane 2: Rat skeletal muscle tissue lysate
  • Western blot analysis of Syntrophin alpha 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Mouse skeletal muscle tissue lysate<br />
Lane 2: Rat skeletal muscle tissue lysate
  • ICC staining of Syntrophin alpha 1 in SW620 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-40, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Flow cytometric analysis of Syntrophin alpha 1 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-40, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of Syntrophin alpha 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse skeletal muscle tissue lysate
Lane 2: Rat skeletal muscle tissue lysate

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Syntrophin alpha 1 Rabbit Polyclonal Antibody (ER1901-40)

Immunogen

Synthetic peptide within mouse syntrophin alpha 1 aa 174-223 / 503.

Host

Rabbit

Positive Control

Mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, SW620, SiHa.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

54 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB:1:500-1:2,000

  • ICC:1:50-1:100

  • FC:1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Syntrophin alpha 1

SYNONYMS

59 kDa dystrophin-associated protein A1 acidic component 1 antibody; Alpha-1-syntrophin antibody; Pro-TGF-alpha cytoplasmic domain-interacting protein 1 antibody; SNT1 antibody; SNT2 antibody; SNT3 antibody; Snta1 antibody; SNTA1_HUMAN antibody; SNTB1 antibody; SNTB2 antibody; Syntrophin alpha 1 antibody; Syntrophin beta 1 antibody; Syntrophin beta 2 antibody; Syntrophin-1 antibody; TACIP1 antibody

SEQUENCE SIMILARITIES

Belongs to the syntrophin family.

TISSUE SPECIFICITY

High expression in skeletal muscle and heart. Low expression in brain, pancreas, liver, kidney and lung. Not detected in placenta.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by CaM-kinase II. Phosphorylation may inhibit the interaction with DMD (By similarity).

SUBCELLULAR LOCATION

Sarcolemma, cytoskeleton, cell junction.

FUNCTION

Syntrophins are cytoplasmic peripheral membrane scaffold proteins that are components of the dystrophin-associated protein complex. This gene is a member of the syntrophin gene family and encodes the most common syntrophin isoform found in cardiac tissues. The N-terminal PDZ domain of this syntrophin protein interacts with the C-terminus of the pore-forming alpha subunit (SCN5A) of the cardiac sodium channel Nav1.5. This protein also associates cardiac sodium channels with the nitric oxide synthase-PMCA4b (plasma membrane Ca-ATPase subtype 4b) complex in cardiomyocytes. This gene is a susceptibility locus for Long-QT syndrome (LQT) - an inherited disorder associated with sudden cardiac death from arrhythmia - and sudden infant death syndrome (SIDS). This protein also associates with dystrophin and dystrophin-related proteins at the neuromuscular junction and alters intracellular calcium ion levels in muscle tissue.