Applications
-
ICC
-
IHC-P
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
SHP-2 Rabbit Polyclonal Antibody (ER1706-93)
Immunogen
Recombinant protein within human shp-2 aa 359-575.
Host
Rabbit
Positive Control
A431, LOVO, MCF-7, rat skeletal muscle tissue, human colon cancer tissue, human fetal skeletal muscle tissue, mouse brain tissue, SH-SY5Y.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
PURIFICATION
Immunogen affinity purified.
MOLECULAR WEIGHT
Predicted band size: 68 kDa
Isotype
IgG
APPLICATION DILUTION
-
ICC
-
1:50-1:200
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
SHP-2
SYNONYMS
BPTP3 antibody;CFC antibody;JMML antibody;METCDS antibody;MGC14433 antibody;NS1 antibody;OTTHUMP00000166107 antibody;OTTHUMP00000166108 antibody;Protein tyrosine phosphatase 2 antibody;Protein tyrosine phosphatase 2C antibody;Protein tyrosine phosphatase non receptor type 11 antibody;Protein-tyrosine phosphatase 1D antibody;Protein-tyrosine phosphatase 2C antibody;PTN11_HUMAN antibody;PTP-1D antibody;PTP-2C antibody;PTP1D antibody;PTP2C antibody;PTPN11 antibody;SAP2 antibody;SH-PTP2 antibody;SH-PTP3 antibody;SH2 domain containing protein tyrosine phosphatase 2 antibody;SHP 2 antibody;SHP-2 antibody;Shp2 antibody;SHPTP2 antibody;SHPTP3 antibody;Syp antibody;Tyrosine-protein phosphatase non-receptor type 11 antibody
SEQUENCE SIMILARITIES
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
TISSUE SPECIFICITY
Widely expressed, with highest levels in heart, brain, and skeletal muscle.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated on Tyr-542 and Tyr-580 upon receptor protein tyrosine kinase activation; which creates a binding site for GRB2 and other SH2-containing proteins. Phosphorylated upon activation of the receptor-type kinase FLT3. Phosphorylated upon activation of the receptor-type kinase PDGFRA (By similarity). Phosphorylated by activated PDGFRB.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
The steady state of protein tyrosyl phosphorylation in cells is regulated by the opposing action of tyrosine kinases and protein tyrosine phosphatases (PTPs). Several groups have independently identified a non-transmembrane PTP, designated SH-PTP1 (also known as PTP1C, HCP and SHP), which is primarily expressed in hematopoietic cells and characterized by the presence of two SH2 domains N-terminal to the PTP domain. SH2 domains generally mediate the association of regulatory molecules with specific phosphotyrosine-containing sites on autophosphorylated receptors, thereby controlling the initial interaction of receptors with these substrates. A second and much more widely expressed PTP with SH2 domains, SH-PTP2 (also designated PTP1D and Syp), has been identified. Strong sequence similarity between SH-PTP2 and the Drosophila gene corkscrew (CSW) and their similar patterns of expression suggest that SH-PTP2 is the human corkscrew homolog.