Serotonin transporter Recombinant Rabbit Monoclonal Antibody [JE34-53]
Catalog# HA721544
Serotonin transporter Recombinant Rabbit Monoclonal Antibody [JE34-53]
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WB
-
FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Serotonin transporter Recombinant Rabbit Monoclonal Antibody [JE34-53]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Serotonin transporter aa 38-87 / 630.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, FC
Molecular Weight
Predicted band size: 70 kDa
Positive Control
Mouse brain tissue lysate, rat brain tissue lysate, mouse lung tissue lysate, HepG2.
Conjugation
unconjugated
Clone Number
JE34-53
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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FC
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1:500-1:1,000
Target
Function
The serotonin transporter (SERT or 5-HTT) also known as the sodium-dependent serotonin transporter and solute carrier family 6 member 4 is a protein that in humans is encoded by the SLC6A4 gene. SERT is a type of monoamine transporter protein that transports the neurotransmitter serotonin from the synaptic cleft back to the presynaptic neuron, in a process known as serotonin reuptake. This transport of serotonin by the SERT protein terminates the action of serotonin and recycles it in a sodium-dependent manner. Many antidepressant medications of the SSRI and tricyclic antidepressant classes work by binding to SERT and thus reducing serotonin reuptake. It is a member of the sodium:neurotransmitter symporter family. A repeat length polymorphism in the promoter of this gene has been shown to affect the rate of serotonin uptake and may play a role in sudden infant death syndrome, aggressive behavior in Alzheimer disease patients, post-traumatic stress disorder and depression-susceptibility in people experiencing emotional trauma.
Background References
1. Gao J et al. Mucosal Serotonin Reuptake Transporter Expression in Irritable Bowel Syndrome Is Modulated by Gut Microbiota Via Mast Cell-Prostaglandin E2. Gastroenterology. 2022 Jun
2. Szoke-Kovacs Z et al. Selective Inhibition of the Serotonin Transporter in the Treatment of Depression: Sertraline, Fluoxetine and Citalopram. Neuropsychopharmacol Hung. 2020 Mar
Subcellular Location
Cell membrane, Endomembrane system, Endosome membrane, Synapse, Cell junction, focal adhesion, Cell projection, neuron projection.
Synonyms
5 HTT antibody
5 HTTLPR antibody
5 hydroxytryptamine (serotonin) transporter antibody
5 hydroxytryptamine transporter antibody
5HT transporter antibody
5HTT antibody
hSERT antibody
HTT antibody
Na+/Cl- dependent serotonin transporter antibody
OCD1 antibody
ExpandImages
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Western blot analysis of Serotonin transporter on different lysates with Rabbit anti-Serotonin transporter antibody (HA721544) at 1/1,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lane 3: Mouse lung tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 70 kDa
Observed band size: 75 kDa
Exposure time: 28 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721544) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Flow cytometric analysis of HepG2 cells labeling Serotonin transporter.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721544, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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