Positive control:
Lane 1: Raji cell lysate
Lane 2: Hela cell lysate
Lane 2: PC-12 cell lysate
Applications
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WB
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ICC
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IF
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IHC-P
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IP
REACTIVITY
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Human
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Mouse
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Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant ATG5 Monoclonal Antibody (ET1611-38)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
Raji cell lysate, Hela cell lysate, PC-12 cell lysate, Hela, MCF-7, human lung carcinoma tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SN73-07
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
55 kDa
Isotype
IgG
APPLICATION DILUTION
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WB
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1:1,000-1:5,000
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ICC/IF
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1:100-1:500
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IHC-P
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1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
ATG5
SYNONYMS
APG 5 antibody; APG 5L antibody; APG5 antibody; APG5 autophagy 5 like antibody; APG5 like antibody; APG5-like antibody; APG5L antibody; Apoptosis specific protein antibody; Apoptosis-specific protein antibody; ASP antibody; ATG 5 antibody; Atg5 antibody; ATG5 autophagy related 5 homolog antibody; ATG5_HUMAN antibody; Autophagy protein 5 antibody; Autophagy related 5 antibody; hAPG5 antibody; Homolog of S Cerevisiae autophagy 5 antibody; OTTHUMP00000040507 antibody
SEQUENCE SIMILARITIES
Belongs to the ATG5 family.
TISSUE SPECIFICITY
Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.
POST-TRANSLATIONAL MODIFICATION
Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.; Acetylated by EP300.
SUBCELLULAR LOCATION
Cytoplasm, Preautophagosomal structure membrane.
FUNCTION
Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.
CITATIONS
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Lin, Cheng et al.
The transcription factor p8 regulates autophagy during diapause embryo formation in Artemia parthenogenetica. | Cell Stress & Chaperones [2016]
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WB
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Artemia
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Citation
-
-
Sheng, Yue et al.
RAB37 interacts directly with ATG5 and promotes autophagosome formation via regulating ATG5-12-16 complex assembly. | Cell Death and Differentiation [2018]
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WB
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human
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Citation
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-
Wang, Wanqiu et al.
Sinomenine attenuates septic-associated lung injury through the Nrf2-Keap1 and autophagy. | The Journal of Pharmacy and Pharmacology [2020]
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WB
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mice
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Citation
-
-
Zhang, Wenkan et al.
Water Extract of Sporoderm-Broken Spores of Ganoderma lucidum Induces Osteosarcoma Apoptosis and Restricts Autophagic Flux. | OncoTargets and Therapy [2019]
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WB
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IHC
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mice
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Citation
-
-
Chen, Lili et al.
Autophagy negative-regulating Wnt signaling enhanced inflammatory osteoclastogenesis from Pre-OCs in vitro. | Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie [2020]
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WB
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human
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Citation
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