Positive control:
Lane 1: human lung tissue lysate
Lane 2: mouse kidney tissue lysate
Lane 3: human liver tissue lysate
Applications
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WB
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IHC-P
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FC
REACTIVITY
-
Human
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Mouse
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant ACE Monoclonal Antibody (ET1705-36)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
Human lung tissue lysate, mouse kidney tissue lysate, human liver tissue lysate, human kidney tissue, mouse testis tissue, mouse kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM59-32
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
160 kDa
Isotype
IgG
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IHC-P
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1:100-1:500
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FC
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1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
ACE
SYNONYMS
ACE 1 antibody; ACE antibody; ACE T antibody; ACE_HUMAN antibody; ACE1 antibody; Angiotensin converting enzyme somatic isoform antibody; Angiotensin converting enzyme testis specific isoform antibody; Angiotensin I converting enzyme 1 antibody; Angiotensin I converting enzyme antibody; Angiotensin I converting enzyme peptidyl dipeptidase A 1 antibody; angiotensin I converting enzyme peptidyl-dipeptidase A 1 transcript antibody; Angiotensin-converting enzyme antibody; Carboxycathepsin antibody; CD 143 antibody; CD143 antibody; CD143 antigen antibody; DCP 1 antibody; DCP antibody; DCP1 antibody; Dipeptidyl carboxypeptidase 1 antibody; Dipeptidyl carboxypeptidase I antibody; Kininase II antibody; MGC26566 antibody; MVCD3 antibody; Peptidase P antibody; Peptidyl dipeptidase A antibody; soluble form antibody; Testicular ECA antibody
SEQUENCE SIMILARITIES
Belongs to the peptidase M2 family.
TISSUE SPECIFICITY
Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated by CK2 on Ser-1299; which allows membrane retention.
SUBCELLULAR LOCATION
Secreted. Cell membrane. Cytoplasm.
FUNCTION
Angiotensin-converting enzyme (ACE) is a carboxy-terminal dipeptidyl exo-peptidase that converts Angiotensin I to the potent vasopressive hormone, Angiotensin II. There are two isoforms of ACE, the pulmonary ACEP and the testicular ACET. ACEP is a glycoprotein expressed in vascular endothelial cells of the lung, liver, adrenal cortex, pancreas, kidney and spleen. The ACET isoform is expressed exclusively in adult testis by developing sperm cells, specifically, late pachytene spermatocytes. Additionally, ACE inactivates bradykinin, a vasodepressor peptide, and is involved in fluid/electrolyte homeostasis. Although it bears significant sequence homology to ACE, ACE2 shows a more restricted pattern of expression. ACE is expressed ubiquitously throughout the vasculature while ACE2 is expressed only in cardiac, renal and testicular cells.
CITATIONS
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Liu, Junjun et al.
Sini decoction alleviates E. coli induced acute lung injury in mice via equilibrating ACE-AngII-AT1R and ACE2-Ang-(1-7)-Mas axis. | Life Sciences [2018]
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WB
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mouse
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Citation
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Chen, Qiuhua et al.
Sini decoction ameliorates sepsis-induced acute lung injury via regulating ACE2-Ang (1-7)-Mas axis and inhibiting the MAPK signaling pathway. | Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie [2019]
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WB
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human
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Citation
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