Raptor Rabbit Polyclonal Antibody (ER1802-57)

UNIPROT #

Q8N122

PROTEIN NAME

Raptor

SYNONYMS

KIAA1303 antibody;KOG1 antibody;Mip1 antibody;P150 target of rapamycin (TOR) scaffold protein antibody;p150 target of rapamycin (TOR) scaffold protein containing WD repeats antibody;P150 target of rapamycin (TOR)-scaffold protein antibody;Raptor antibody;Regulatory associated protein of mTOR antibody;Regulatory associated protein of MTOR complex 1 antibody;Regulatory-associated protein of mTOR antibody;RPTOR antibody;RPTOR_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the WD repeat RAPTOR family.

TISSUE SPECIFICITY

Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. Isoform 3 is widely expressed, with highest levels in nasal mucosa and pituitary and lowest in spleen.

POST-TRANSLATIONAL MODIFICATION

Insulin-stimulated phosphorylation at Ser-863 by MTOR and MAPK8 up-regulates mTORC1 activity. Osmotic stress also induces phosphorylation at Ser-696, Thr-706 and Ser-863 by MAPK8. Ser-863 phosphorylation is required for phosphorylation at Ser-855 and Ser-859. In response to nutrient limitation, phosphorylated by AMPK; phosphorylation promotes interaction with 14-3-3 proteins, leading to negative regulation of the mTORC1 complex. In response to growth factors, phosphorylated at Ser-719, Ser-721 and Ser-722 by RPS6KA1, which stimulates mTORC1 activity.

SUBCELLULAR LOCATION

Cytoplasm. Lysosome.

FUNCTION

Regulatory associated protein of FRAP, also designated Raptor, is a binding partner for mammalian target of rapamycin kinase (FRAP), and is essential for FRAP signalling in vivo. Raptor binding to FRAP is critical for FRAP-catalysed substrate phosphorylation of 4E-BP1. The raptor-FRAP complex is nutrient-sensitive and is important for a mechanism by which cells coordinate cell growth and size with changing environmental conditions. Raptor serves as a negative regulator of FRAP kinase activity under nutrient-deprived conditions and is an important component in the FRAP pathway. Raptor is highly expressed in skeletal muscle and to a lesser extent in brain, kidney, lung and placenta.