PRODUCT CODE: ER1802-57

Raptor Rabbit Polyclonal Antibody (ER1802-57)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of Raptor on MCF-7 cell lysate using anti-Raptor antibody at 1/500 dilution.
  • Western blot analysis of Raptor on MCF-7 cell lysate using anti-Raptor antibody at 1/500 dilution.
  • ICC staining Raptor in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Raptor in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Raptor antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Raptor antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Raptor antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of A549 cells with Raptor antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Raptor on MCF-7 cell lysate using anti-Raptor antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Raptor Rabbit Polyclonal Antibody (ER1802-57)

Immunogen

Synthetic peptide within human raptor aa 100-150.

Host

Rabbit

Positive Control

MCF-7, 293T, LOVO, rat brain tissue, human breast tissue, mouse colon tissue, A549.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

131 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Raptor

SYNONYMS

KIAA1303 antibody; KOG1 antibody; Mip1 antibody; P150 target of rapamycin (TOR) scaffold protein antibody; p150 target of rapamycin (TOR) scaffold protein containing WD repeats antibody; P150 target of rapamycin (TOR)-scaffold protein antibody; Raptor antibody; Regulatory associated protein of mTOR antibody; Regulatory associated protein of MTOR complex 1 antibody; Regulatory-associated protein of mTOR antibody; RPTOR antibody; RPTOR_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the WD repeat RAPTOR family.

TISSUE SPECIFICITY

Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. Isoform 3 is widely expressed, with highest levels in nasal mucosa and pituitary and lowest in spleen.

POST-TRANSLATIONAL MODIFICATION

Insulin-stimulated phosphorylation at Ser-863 by MTOR and MAPK8 up-regulates mTORC1 activity. Osmotic stress also induces phosphorylation at Ser-696, Thr-706 and Ser-863 by MAPK8. Ser-863 phosphorylation is required for phosphorylation at Ser-855 and Ser-859. In response to nutrient limitation, phosphorylated by AMPK; phosphorylation promotes interaction with 14-3-3 proteins, leading to negative regulation of the mTORC1 complex. In response to growth factors, phosphorylated at Ser-719, Ser-721 and Ser-722 by RPS6KA1, which stimulates mTORC1 activity.

SUBCELLULAR LOCATION

Cytoplasm. Lysosome.

FUNCTION

Regulatory associated protein of FRAP, also designated Raptor, is a binding partner for mammalian target of rapamycin kinase (FRAP), and is essential for FRAP signalling in vivo. Raptor binding to FRAP is critical for FRAP-catalysed substrate phosphorylation of 4E-BP1. The raptor-FRAP complex is nutrient-sensitive and is important for a mechanism by which cells coordinate cell growth and size with changing environmental conditions. Raptor serves as a negative regulator of FRAP kinase activity under nutrient-deprived conditions and is an important component in the FRAP pathway. Raptor is highly expressed in skeletal muscle and to a lesser extent in brain, kidney, lung and placenta.