PUMA Recombinant Rabbit Monoclonal Antibody [SR42-09] (ET1602-24)
Catalog# ET1602-24
Rabbit Monoclonal to PUMA
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: HDLM-2 cell lysate
Lane 4: NIH/3T3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 24 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-24) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
PUMA Recombinant Rabbit Monoclonal Antibody [SR42-09] (ET1602-24)
Immunogen
Synthetic peptide within human puma aa 153-193 / 193.
Host
Rabbit
Positive Control
HeLa cell lysate, HepG2 cell lysate, HDLM-2 cell lysate, NIH/3T3 cell lysate, Hela, SKOV-3, human stomach cancer tissue, mouse small intestine tissue, rat small intestine tissue, rat stomach tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SR42-09
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IF-Cell
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1:50
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IF-Tissue
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1:200-1:500
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IHC-P
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1:1,000-1:5,000
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FC
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1:50
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 21 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
PUMA
SYNONYMS
BBC 3 antibody;Bbc3 antibody;BBC3_HUMAN antibody;BCL 2 binding component 3 antibody;Bcl-2-binding component 3 antibody;BCL2 binding component 3 antibody;JFY 1 antibody;JFY-1 antibody;JFY1 antibody;p53 up regulated modulator of apoptosis antibody;p53 up-regulated modulator of apoptosis antibody;p53 Upregulated Modulator of Apoptosis antibody;PUMA alpha antibody;PUMA/JFY1 antibody
SEQUENCE SIMILARITIES
Belongs to the Bcl-2 family.
TISSUE SPECIFICITY
Ubiquitously expressed.
SUBCELLULAR LOCATION
Mitochondrion.
FUNCTION
The p53 upregulated modulator of apoptosis (PUMA) also known as Bcl-2-binding component 3 (BBC3), is a pro-apoptotic protein, member of the Bcl-2 protein family. In humans, the Bcl-2-binding component 3 protein is encoded by the BBC3 gene. The expression of PUMA is regulated by the tumor suppressor p53. PUMA is involved in p53-dependent and -independent apoptosis induced by a variety of signals, and is regulated by transcription factors, not by post-translational modifications. After activation, PUMA interacts with antiapoptotic Bcl-2 family members, thus freeing Bax and/or Bak which are then able to signal apoptosis to the mitochondria. Following mitochondrial dysfunction, the caspase cascade is activated ultimately leading to cell death.
CITATIONS
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Wei Zheng,et al
Poly (lactic acid hydroxyacetic acid)-poly (ethylene glycol)-modified ginsenoside Rg3 nanomedicine enhances anti-tumor effect in hepatocellular carcinoma
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WB
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Mouse
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Citation
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Wan, T., Zhong, J., Pan, Q., Zhou, T., Ping, Y., & Liu, X.
Exosome-mediated delivery of Cas9 ribonucleoprotein complexes for tissue-specific gene therapy of liver diseases
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WB
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Mouse
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Citation
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