PRODUCT CODE: ER1802-10

PSMA Rabbit Polyclonal Antibody (ER1802-10)

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

Western blot analysis of PSMA on human small intestine tissue lysate using anti-PSMA antibody at 1/1,000 dilution.
  • Western blot analysis of PSMA on human small intestine tissue lysate using anti-PSMA antibody at 1/1,000 dilution.
  • ICC staining PSMA in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PSMA in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of HepG2 cells with PSMA antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of PSMA on human small intestine tissue lysate using anti-PSMA antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

PSMA Rabbit Polyclonal Antibody (ER1802-10)

Immunogen

Synthetic peptide within human psma aa 684-726.

Host

Rabbit

Positive Control

Human small intestine tissue lysate, HepG2, PC-3M.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

50/85 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

PSMA

SYNONYMS

Cell growth inhibiting protein 27 antibody; Cell growth-inhibiting gene 27 protein antibody; FGCP antibody; Folate hydrolase (prostate-specific membrane antigen) 1 antibody; Folate hydrolase 1 antibody; Folate hydrolase antibody; Folate hydrolase prostate specific membrane antigen 1 antibody; FOLH 1 antibody; FOLH antibody; Folh1 antibody; FOLH1_HUMAN antibody; Folylpoly gamma glutamate carboxypeptidase antibody; Folylpoly-gamma-glutamate carboxypeptidase antibody; GCP 2 antibody; GCP II antibody; GCP2 antibody; GCPII antibody; GIG27 antibody; Glutamate carboxylase II antibody; Glutamate carboxypeptidase 2 antibody; Glutamate carboxypeptidase II antibody; Membrane glutamate carboxypeptidase antibody; mGCP antibody; N acetylated alpha linked acidic dipeptidase 1 antibody; N-acetylated-alpha-linked acidic dipeptidase I antibody; NAALAD 1 antibody; NAALAD1 antibody; NAALAdase antibody; NAALADase I antibody; Prostate specific membrane antigen antibody; Prostate specific membrane antigen variant F antibody; Prostate-specific membrane antigen antibody; PSM antibody; PSMA antibody; Pteroylpoly gamma glutamate carboxypeptidase antibody; Pteroylpoly-gamma-glutamate carboxypeptidase antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase M28 family. M28B subfamily.

TISSUE SPECIFICITY

Highly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-3 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer.

POST-TRANSLATIONAL MODIFICATION

The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.; The N-terminus is blocked.

SUBCELLULAR LOCATION

Cytoplasm. Cell membrane.

FUNCTION

Prostate cancer is the most frequently diagnosed cancer and the early detection of prostate cancer dramatically and efficiently reduces the observed mortality rate. Several proteins have been identified as specific markers of prostate cancer, and they may be useful as diagnostic indicators. PSA, prostate specific antigen, is the classical indicator for transformed prostate tissue; however, in addition to being upregulated in prostate cancer, PSA is also upregulated in non-malignant conditions, such as benign prostatic hyperplasia prostate. Conversely, STEAP (six-transmembrane epithelial antigen of the prostate), prostate carcinoma tumor antigen (PCTA-1) and prostate-specific membrane antigen (PSM) represent additional prostate-specific antigens that are overexpressed only in malignant tumors and therefore are more specific identifiers of malignancies. PSM is an integral membrane protein, and PCTA-1 is related to the galectin gene family, which mediate both cell-cell and cell-matrix interactions in a manner similar to the selectin subgroup of C-type lectins. STEAP is a serpentine transmembrane cell-surface tumor-antigen that is predicted to functions as a channel or transporter protein.