Lysates/proteins at 50 ng/Lane.
Exposure time: 1 minute;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1005-2) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Applications
-
WB
-
ICC
-
IHC-P
REACTIVITY
-
Human
-
Mouse
SPECIFICATIONS
Product Type
Mouse monoclonal primary
Product Name
Protein FAM38A Mouse Monoclonal Antibody [2-10] (M1005-2)
Immunogen
Recombinant protein within human protein fam38a aa 1275-1540 / 2521.
Host
Mouse
Positive Control
Recombinant protein, Mouse brain tissue, A431, rat brain tissue, rat hippocampus tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
2-10
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
Predicted band size: 287 kDa
Isotype
IgG2a
APPLICATION DILUTION
-
WB
-
1:500-1:2,000
-
ICC
-
1:100
-
IHC-P
-
1:200-1:600
TARGET
UNIPROT #
PROTEIN NAME
Protein FAM38A
SYNONYMS
DHS;Fam38a;Family with sequence similarity 38 member A;KIAA0233;Membrane protein induced by beta-amyloid treatment;Mib;PIEZ1_HUMAN;Piezo-type mechanosensitive ion channel component 1;PIEZO1;Protein FAM38A;Protein FAM38B;Protein PIEZO1
SEQUENCE SIMILARITIES
Belongs to the PIEZO (TC 1.A.75) family.
TISSUE SPECIFICITY
Expressed in numerous tissues. In normal brain, expressed exclusively in neurons, not in astrocytes. In Alzheimer disease brains, expressed in about half of the activated astrocytes located around classical senile plaques. In Parkinson disease substantia nigra, not detected in melanin-containing neurons nor in activated astrocytes. Expressed in erythrocytes (at protein level).
DEVELOPMENTAL STAGE
At 17 weeks of gestation, strongly expressed in hepatic erythroblasts. At that stage, also expressed in fetal splenic plasma cells and in lymphatic vessel of fetal peritoneum. In vitro, up-regulated during the erythroid differentiation of CD34+ cells from healthy donors (at protein level).
SUBCELLULAR LOCATION
Endoplasmic reticulum membrane. Cell membrane.
FUNCTION
Pore-forming subunit of a mechanosensitive non-specific cation channel. Generates currents characterized by a linear current-voltage relationship that are sensitive to ruthenium red and gadolinium. Plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER, most probably in its activated state, and subsequent stimulation of calpain signaling. In the kidney, may contribute to the detection of intraluminal pressure changes and to urine flow sensing. Acts as shear-stress sensor that promotes endothelial cell organization and alignment in the direction of blood flow through calpain activation. Plays a key role in blood vessel formation and vascular structure in both development and adult physiology.