PRODUCT CODE: M1005-2

Protein FAM38A Mouse Monoclonal Antibody [2-10] (M1005-2)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

Western blot analysis of FAM38A/PIEZO1 on recombinant protein with Mouse anti-FAM38A/PIEZO1 antibody (M1005-2) at 1/500 dilution.<br />
<br />
Lysates/proteins at 50 ng/Lane.<br />
<br />
Exposure time: 1 minute;<br />
<br />
10% SDS-PAGE gel.<br />
<br />
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1005-2) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of FAM38A/PIEZO1 on recombinant protein with Mouse anti-FAM38A/PIEZO1 antibody (M1005-2) at 1/500 dilution.<br />
<br />
Lysates/proteins at 50 ng/Lane.<br />
<br />
Exposure time: 1 minute;<br />
<br />
10% SDS-PAGE gel.<br />
<br />
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1005-2) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-FAM38A/PIEZO1 antibody (M1005-2) at 1/200 dilution.<br />
<br />
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1005-2) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunocytochemistry analysis of A431 cells labeling FAM38A/PIEZO1 with Mouse anti-FAM38A/PIEZO1 antibody (M1005-2) at 1/100 dilution.<br />
<br />
Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-FAM38A/PIEZO1 antibody (M1005-2) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-FAM38A/PIEZO1 antibody (M1005-2) at 1/600 dilution.<br />
<br />
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1005-2) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Mouse anti-FAM38A/PIEZO1 antibody (M1005-2) at 1/600 dilution.<br />
<br />
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1005-2) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of FAM38A/PIEZO1 on recombinant protein with Mouse anti-FAM38A/PIEZO1 antibody (M1005-2) at 1/500 dilution.

Lysates/proteins at 50 ng/Lane.

Exposure time: 1 minute;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1005-2) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Protein FAM38A Mouse Monoclonal Antibody [2-10] (M1005-2)

Immunogen

Recombinant protein within human protein fam38a aa 1275-1540 / 2521.

Host

Mouse

Positive Control

Recombinant protein, Mouse brain tissue, A431, rat brain tissue, rat hippocampus tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

2-10

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

Predicted band size: 287 kDa

Isotype

IgG2a

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:100

  • IHC-P

  • 1:200-1:600

TARGET

UNIPROT #

PROTEIN NAME

Protein FAM38A

SYNONYMS

DHS;Fam38a;Family with sequence similarity 38 member A;KIAA0233;Membrane protein induced by beta-amyloid treatment;Mib;PIEZ1_HUMAN;Piezo-type mechanosensitive ion channel component 1;PIEZO1;Protein FAM38A;Protein FAM38B;Protein PIEZO1

SEQUENCE SIMILARITIES

Belongs to the PIEZO (TC 1.A.75) family.

TISSUE SPECIFICITY

Expressed in numerous tissues. In normal brain, expressed exclusively in neurons, not in astrocytes. In Alzheimer disease brains, expressed in about half of the activated astrocytes located around classical senile plaques. In Parkinson disease substantia nigra, not detected in melanin-containing neurons nor in activated astrocytes. Expressed in erythrocytes (at protein level).

DEVELOPMENTAL STAGE

At 17 weeks of gestation, strongly expressed in hepatic erythroblasts. At that stage, also expressed in fetal splenic plasma cells and in lymphatic vessel of fetal peritoneum. In vitro, up-regulated during the erythroid differentiation of CD34+ cells from healthy donors (at protein level).

SUBCELLULAR LOCATION

Endoplasmic reticulum membrane. Cell membrane.

FUNCTION

Pore-forming subunit of a mechanosensitive non-specific cation channel. Generates currents characterized by a linear current-voltage relationship that are sensitive to ruthenium red and gadolinium. Plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER, most probably in its activated state, and subsequent stimulation of calpain signaling. In the kidney, may contribute to the detection of intraluminal pressure changes and to urine flow sensing. Acts as shear-stress sensor that promotes endothelial cell organization and alignment in the direction of blood flow through calpain activation. Plays a key role in blood vessel formation and vascular structure in both development and adult physiology.