Proteasome 20S beta 6 Rabbit Polyclonal Antibody
Catalog# HA500349
Proteasome 20S beta 6 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Proteasome 20S beta 6 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within Human Proteasome 20S beta 6 aa 21-239 / 239.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, FC
Molecular Weight
Predicted band size: 25 kDa
Positive Control
C2C12 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Hela cell lysate, Jurkat cell lysate, Mouse colon tissue lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate, HeLa, C6, NIH/3T3.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:5,000
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IF-Cell
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1:200
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FC
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1:500-1:1,000
Target
Function
In eukaryotic cells, selective breakdown of cellular proteins is ensured by their ubiquitination and subsequent degradation by the 26S Proteasome. The 26S Proteasome is a protease complex that selectively breaks down proteins that have been modified by polyubiquitin chains. It is made up of two multisubunit complexes: the 20S Proteasome chamber, which serves as the proteolytic core of the complex and two 19S regulatory particles which recognize and unfold ubiquitinated proteins. The 20S Proteasome chamber contains α subunits (which are structural) and β subunits (which are predominantly catalytic). The outer two rings in the proteasome consist of seven α subunits each, and the inner two rings each consist of seven β subunits. PSMB6 (proteasome (prosome, macropain) subunit, β type, 6), also known as LMPY (PSY large multifunctional protease Y), macropain δ chain, proteasome δ chain or proteasome subunit Y, is a β subunit of the 20S Proteasome and, upon stimulation with IFN-γ, can be displaced by LMP2.
Background References
1. Huang WR. et. al. Avian reovirus p17 and σA act cooperatively to downregulate Akt by suppressing mTORC2 and CDK2/cyclin A2 and upregulating proteasome PSMB6. Sci Rep. 2017 Jul
2. Sun L. et. al. Identification of proteasome subunit beta type 6 (PSMB6) associated with deltamethrin resistance in mosquitoes by proteomic and bioassay analyses. PLoS One. 2013 Jun
Subcellular Location
Cytoplasm, Nucleus.
Synonyms
DELTA antibody
LMPY antibody
Macropain delta chain antibody
MGC5169 antibody
Multicatalytic endopeptidase complex delta chain antibody
Proteasome (prosome macropain) subunit beta type 6 antibody
Proteasome beta 6 subunit antibody
Proteasome catalytic subunit 1 antibody
Proteasome delta chain antibody
Proteasome subunit beta 6 antibody
ExpandImages
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Western blot analysis of Proteasome 20S beta 6 on different lysates with Rabbit anti-Proteasome 20S beta 6 antibody (HA500349) at 1/5,000 dilution.
Lane 1: C2C12 cell lysate
Lane 2: NIH/3T3 cell lysate
Lane 3: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500349) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Proteasome 20S beta 6 on different lysates with Rabbit anti-Proteasome 20S beta 6 antibody (HA500349) at 1/500 dilution.
Lane 1: Hela cell lysate (10 µg/Lane)
Lane 2: Jurkat cell lysate (10 µg/Lane)
Lane 3: Mouse colon tissue lysate (20 µg/Lane)
Lane 4: Mouse kidney tissue lysate (20 µg/Lane)
Lane 5: Rat kidney tissue lysate (20 µg/Lane)
Predicted band size: 25 kDa
Observed band size: 23/25 kDa
Exposure time: 1 minute;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500349) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HeLa cells labeling Proteasome 20S beta 6 with Rabbit anti-Proteasome 20S beta 6 antibody (HA500349) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Proteasome 20S beta 6 antibody (HA500349) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of C6 cells labeling Proteasome 20S beta 6 with Rabbit anti-Proteasome 20S beta 6 antibody (HA500349) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Proteasome 20S beta 6 antibody (HA500349) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of NIH/3T3 cells labeling Proteasome 20S beta 6 with Rabbit anti-Proteasome 20S beta 6 antibody (HA500349) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Proteasome 20S beta 6 antibody (HA500349) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
Flow cytometric analysis of HeLa cells labeling Proteasome 20S beta 6.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA500349, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Flow cytometric analysis of NIH/3T3 cells labeling Proteasome 20S beta 6.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA500349, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Flow cytometric analysis of C6 cells labeling Proteasome 20S beta 6.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA500349, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"