Phospho-RSK1 p90 (T359+S363) Recombinant Rabbit Monoclonal Antibody [SU03-65] (ET1608-53)
Catalog# ET1608-53
Rabbit Monoclonal to Phospho-RSK1 p90 (T359+S363)
Tested for
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WB
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IF-Cell
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IHC-P
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IP
React with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Phospho-RSK1 p90(T359+S363) on different lysates with Rabbit anti-Phospho-RSK1 p90(T359+S363) antibody (ET1608-53) at 1/500 dilution.
Lane 1: MCF-7 cell lysate
Lane 2: A431 cell lysate
Lane 3: 293 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 83 kDa
Observed band size: 83 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-53) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Lane 1: MCF-7 cell lysate
Lane 2: A431 cell lysate
Lane 3: 293 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 83 kDa
Observed band size: 83 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-53) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
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