PRODUCT CODE: ER1802-51

Pax8 Rabbit Polyclonal Antibody (ER1802-51)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of Pax8 on human thyroid gland tissue and SKOV-3 cell lysates using anti-Pax8 antibody at 1/500 dilution.
  • Western blot analysis of Pax8 on human thyroid gland tissue and SKOV-3 cell lysates using anti-Pax8 antibody at 1/500 dilution.
  • ICC staining Pax8 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-Pax8 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SiHa cells with Pax8 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Pax8 on human thyroid gland tissue and SKOV-3 cell lysates using anti-Pax8 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Pax8 Rabbit Polyclonal Antibody (ER1802-51)

Immunogen

Synthetic peptide within c-terminal human pax8.

Host

Rabbit

Positive Control

SKOV-3, human thyroid gland tissue, SiHa.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

48 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Pax8

SYNONYMS

OTTHUMP00000158659 antibody; OTTHUMP00000158660 antibody; OTTHUMP00000203723 antibody; OTTHUMP00000203724 antibody; Paired box 8 antibody; Paired box gene 8 antibody; paired box homeotic gene 8 antibody; Paired box protein Pax 8 antibody; Paired box protein Pax-8 antibody; Paired domain gene 8 antibody; PAX 8 antibody; PAX8 antibody; PAX8_HUMAN antibody

TISSUE SPECIFICITY

Expressed in the excretory system, thyroid gland and Wilms tumors.

DEVELOPMENTAL STAGE

In developing excretory system, during thyroid differentiation and in adult thyroid.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

The Pax family encodes transcription factors that function during embryogenesis and regulate the temporal and position-dependent differentiation of cells. Pax-8 is expressed in the developing and adult thyroid, the developing secretory system and at lower levels, in the adult kidney. Pax-8 complexes with TTF-1 and TTF-2 to induce thyroid follicular cell differentiation and thyroid hormone biosynthesis by regulating the expression of sodium iodide symporter (NIS), thyroid peroxidase (TPO), thyroglobulin (TG) and the thyrotropin receptor (TSHR). Treatment of FRTL-5 cells with TGFβ1 decreases Pax-8 mRNA levels and Pax-8 DNA binding activity, which suppresses the expression of TG and the formation of thyrocytes. Patients who have autosomal dominant mutations of the Pax-8 gene develop thyroid dysgenesis. The Pax-8 gene produces six isoforms, A to F, that are generated by alternative splicing and differ in their carboxy-terminal regions. The Pax-8 isoforms display different DNA binding capacities and are thought to be functionally distinct. The gene which encodes Pax-8 maps to human chromosome 2q12-q14.