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p70 S6 Kinase beta Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER31205

p70 S6 Kinase beta Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

p70 S6 Kinase beta Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within human p70 S6 Kinase beta aa 411-460.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P, FC

Molecular Weight

Predicted band size: 59 kDa

Positive Control

HeLa cell lysate, NIH/3T3 cell lysate, C6 cell lysate, HeLa, human pancreas tissue, HepG2.

Conjugation

unconjugated

RRID

AB_3069560

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:200

  • IHC-P

  • 1:5,000

  • FC

  • 1:100-1:200

Target

Function

Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex.

Background References

1. "Regulation of ribosomal S6 kinase 2 by mammalian target of rapamycin." Park I.H., Bachmann R., Shirazi H., Chen J. J. Biol. Chem. 277:31423-31429(2002)

2. "Protein kinase C phosphorylates ribosomal protein S6 kinase betaII and regulates its subcellular localization." Valovka T., Verdier F., Cramer R., Zhyvoloup A., Fenton T., Rebholz H., Wang M.L., Gzhegotsky M., Lutsyk A., Matsuka G., Filonenko V., Wang L., Proud C.G., Parker P.J., Gout I.T. Mol. Cell. Biol. 23:852-863(2003)

Sequence Similarity

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.

Tissue Specificity

Widely expressed.

Post-translational Modification

Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism (By similarity). Activated by phosphorylation at Thr-252 by PDPK1. Dephosphorylation by PPP1CC at Thr-412 in mitochondrion.

Subcellular Location

Cytoplasm, Nucleus

UNIPROT

SwissProt: P23443 Human

SwissProt: Q8BSK8 Mouse

SwissProt: P67999 Rat

Synonyms

70 kDa ribosomal protein S6 kinase 1 antibody

KS6B1_HUMAN antibody

p70 alpha antibody

P70 beta 1 antibody

p70 ribosomal S6 kinase alpha antibody

p70 ribosomal S6 kinase beta 1 antibody

p70 S6 kinase alpha antibody

P70 S6 Kinase antibody

p70 S6 kinase, alpha 1 antibody

p70 S6 kinase, alpha 2 antibody

Expand

Images

  • Western blot analysis of p70 S6 Kinase beta on different lysates with Rabbit anti-p70 S6 Kinase beta antibody (<a href="/products/ER31205" style="font-weight: bold;text-decoration: underline;">ER31205</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: NIH/3T3 cell lysate<br />Lane 3: C6 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 59 kDa<br />Observed band size: 59 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER31205" style="font-weight: bold;text-decoration: underline;">ER31205</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of p70 S6 Kinase beta on different lysates with Rabbit anti-p70 S6 Kinase beta antibody (ER31205) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: NIH/3T3 cell lysate
    Lane 3: C6 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 59 kDa
    Observed band size: 59 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER31205) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-p70 S6 Kinase beta antibody (<a href="/products/ER31205" style="font-weight: bold;text-decoration: underline;">ER31205</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER31205" style="font-weight: bold;text-decoration: underline;">ER31205</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-p70 S6 Kinase beta antibody (ER31205) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER31205) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ICC staining p70 S6 Kinase beta in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining p70 S6 Kinase beta in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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