p50 dynamitin Rabbit Polyclonal Antibody
Catalog# HA500029
p50 dynamitin Rabbit Polyclonal Antibody
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WB
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IF-Cell
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FC
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Human
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Mouse
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unconjugated
Overview
Product Name
p50 dynamitin Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human p50 dynamitin aa 1-200.
Species Reactivity
Human, Mouse
Validated Applications
WB, IF-Cell, FC
Molecular Weight
Predicted band size: 44 kDa
Positive Control
SHSY5Y cell lysate, Mouse brain tissue lysate, SH-SY5Y.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IF-Cell
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1:200
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FC
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1:1,000
Target
Function
This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 23 subunits (11 individual proteins ranging in size from 22 to 150 kD). The subunit is commonly referred to as p50 or dynamitin. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that bind to two copies of p150-glued (DCTN1) and two copies of p24 (DCTN3) to form the dynactin shoulder domain.
Background References
1. Gevaert K. et. al. Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides. Nat. Biotechnol. 21:566-569(2003).
Subcellular Location
Cytoplasm, Cytoskeleton, Dynein, Membrane, Microtubule.
Synonyms
50 kD dynein associated polypeptide antibody
50 kDa dynein associated polypeptide antibody
50 kDa dynein-associated polypeptide antibody
DCTN-50 antibody
DCTN2 antibody
DCTN2_HUMAN antibody
DCTN50 antibody
dynactin 2 (p50) antibody
dynactin 2 antibody
dynactin complex 50 kD subunit antibody
ExpandImages
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Western blot analysis of p50 dynamitin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500029, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SHSY5Y cell lysate
Lane 2: Mouse brain tissue lysate -
Immunocytochemistry analysis of SH-SY5Y cells labeling p50 dynamitin with Rabbit anti-p50 dynamitin antibody (HA500029) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p50 dynamitin antibody (HA500029) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of SH-SY5Y cells labeling p50 dynamitin.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA500029, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"