PRODUCT CODE: ET7108-84

ORP150 Recombinant Rabbit Monoclonal Antibody [JG39-11] (ET7108-84)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of ORP150 on mouse prostate tissue lysate using anti-ORP150 antibody at 1/5,000 dilution.
  • Western blot analysis of ORP150 on mouse prostate tissue lysate using anti-ORP150 antibody at 1/5,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-ORP150 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ORP150 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-ORP150 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ORP150 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with ORP150 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).  Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of ORP150 on mouse prostate tissue lysate using anti-ORP150 antibody at 1/5,000 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

ORP150 Recombinant Rabbit Monoclonal Antibody [JG39-11] (ET7108-84)

Immunogen

Recombinant protein within human orp150 aa 800-950.

Host

Rabbit

Positive Control

HepG2, mouse prostate tissue lysate, rat testis tissue, human liver cancer tissue, human small intestine tissue, mouse testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JG39-11

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

111 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:5,000-1:20,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

ORP150

SYNONYMS

150 kDa oxygen regulated protein antibody; 150 kDa oxygen-regulated protein antibody; 170 kDa glucose regulated protein antibody; 170 kDa glucose-regulated protein antibody; DKFZp686N08236 antibody; FLJ94899 antibody; FLJ97572 antibody; glucose regulated protein 170 antibody; Grp 170 antibody; GRP-170 antibody; Grp170 antibody; HSP 12A antibody; HSP12A antibody; Hyou1 antibody; HYOU1_HUMAN antibody; Hypoxia up regulated 1 antibody; hypoxia up-regulated 1 precursor antibody; Hypoxia up-regulated protein 1 antibody; Hypoxia upregulated 1 antibody; Orp 150 antibody; ORP-150 antibody; Orp150 antibody; oxygen regulated protein (150kD) antibody

SEQUENCE SIMILARITIES

Belongs to the heat shock protein 70 family.

TISSUE SPECIFICITY

Highly expressed in tissues that contain well-developed endoplasmic reticulum and synthesize large amounts of secretory proteins. Highly expressed in liver and pancreas and lower expression in brain and kidney. Also expressed in macrophages within aortic atherosclerotic plaques, and in breast cancers.

SUBCELLULAR LOCATION

Endoplasmic reticulum.

FUNCTION

ORP150, also known as HYOU1 (hypoxia upregulated 1), is a 999 amino acid protein that localizes to the lumen of the endoplasmic reticulum (ER) and is a member of the heat shock protein 70 (HSP 70) family. ORP150 is highly expressed in tissues that have well-developed ERs and a large number of secretory proteins (such as liver and pancreas). It is expressed at lower levels in kidney and brain, and plays an essential role in cytoprotective cellular responses to hypoxia (oxygen deprivation). Specifically, ORP150 participates in protein folding and secretion in the ER and functions to protect cells from hypoxia-induced apoptosis, thereby playing a crucial role in cell survival. ORP150 expression is upregulated in a variety of tumors, such as breast cancer, suggesting an important role in tumorigenesis. The gene encoding ORP150 has two translation initiation sites, resulting in a truncated transcript that lacks an ER signal peptide, but is thought to function as a housekeeping protein in the cytoplasm.