NUR77 Recombinant Rabbit Monoclonal Antibody [JM59-11]
Overview
Product Name
NUR77 Recombinant Rabbit Monoclonal Antibody [JM59-11]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human NUR77 aa 10-49 / 598.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IF-Tissue, IHC-P, FC
Molecular Weight
Predicted band size: 64 kDa
Positive Control
Rat brain tissue lysates, HepG2, Hela, NIH/3T3, human liver tissue, human colon carcinoma tissue, human breast tissue, mouse testis tissue, mouse ovarian tissue.
Conjugation
unconjugated
Clone Number
JM59-11
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000-1:5,000
-
IF-Cell
-
1:50-1:200
-
IF-Tissue
-
1:50-1:200
-
IHC-P
-
1:5,000
-
FC
-
1
-
1,000
Target
Function
Nurr1 (Nur-related factor 1) and Nur77 (also designated NGFI-B) encode orphan nuclear receptors which may comprise an additional subfamily within the nuclear receptor superfamily. The rat and human homologs of mouse Nurr1 are designated RNR1 and NOT, respectively. Both Nurr1 and Nur77 are growth factor inducible immediate early response genes. Induction of both Nurr1 and Nur77 is seen after membrane depolarization while only Nur77 induction is seen with NGF stimulation. JunD acts as a mediator for Nur77. An increase in Nur77 expression is seen in activated T cells during G0 to G1 transition and throughout the G1 phase. In addition to its function as an immediate early gene, Nur77 may play a role in TCR-mediated apoptosis. Cyclosporin A, a potent immunosuppressant, has been shown to inhibit the ability of Nur77 to bind DNA. A dominant negative form of Nur77 can protect T cell hybridomas from activation-induced apoptosis. However, the absolute requirement of Nur77 for TCR-mediated apoptosis is still under debate.
Background References
1. Liu NA et al. Cyclin E - mediated human proopiomelanocortin regulation as a therapeutic target for Cushing disease. J Clin Endocrinol Metab N/A:jc20151606 (2015).
2. Yu Y et al. The orphan nuclear receptor Nur77 inhibits low shear stress-induced carotid artery remodeling in mice. Int J Mol Med 36:1547-55 (2015).
Sequence Similarity
Belongs to the nuclear hormone receptor family. NR4 subfamily.
Tissue Specificity
Fetal muscle and adult liver, brain and thyroid.
Post-translational Modification
Phosphorylated at Ser-351 by RPS6KA1 and RPS6KA3 in response to mitogenic or stress stimuli.; Acetylated by p300/CBP, acetylation increases stability. Deacetylated by HDAC1.
Subcellular Location
Cytoplasm. Nucleus. Nucleus membrane.
Synonyms
Early response protein NAK1 antibody
GFRP 1 antibody
GFRP antibody
GFRP1 antibody
Growth factor inducible nuclear protein N10 antibody
Growth Factor Inducible Nuclear Protein NP10 antibody
Growth Factor Response Protein 1 antibody
Hbr1 antibody
HMR antibody
Hormone Receptor antibody
ExpandImages
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Western blot analysis of NUR77 on different lysates with Rabbit anti-NUR77 antibody (ET1703-97) at 1/5,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Rat brain tissue lysate
Lysates/proteins at 20,40 µg/Lane.
Predicted band size: 64 kDa
Observed band size: 64 kDa
Exposure time: 3 minutes
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-97) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-NUR77 antibody (ET1703-97) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-97) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-NUR77 antibody (ET1703-97) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-97) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-NUR77 antibody (ET1703-97) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-97) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-NUR77 antibody (ET1703-97) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-97) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of HepG2 cells labeling NUR77 with Rabbit anti-NUR77 antibody (ET1703-97) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NUR77 antibody (ET1703-97) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of PC-12 cells labeling NUR77 with Rabbit anti-NUR77 antibody (ET1703-97) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NUR77 antibody (ET1703-97) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of HepG2 cells labeling NUR77.
Cells were fixed and permeabilized. Then stained with the primary antibody (ET1703-97, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Flow cytometric analysis of PC-12 cells labeling NUR77.
Cells were fixed and permeabilized. Then stained with the primary antibody (ET1703-97, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
-
Microglial Nr4a1 deficiency and neuronal C3 deposition mediate TMJ inflammation-induced hippocampal excessive synaptic pruning and depression-like behaviors in mice
Journal: Brain, Behavior, And Immunity
DOI: 10.1016/j.bbi.2025.106128
IF: 7.6
Application: IF-tissue,WB
Reactivity: Mouse
Publish date: 2025 Oct
-
Metformin inhibits heterotopic ossification of mouse Achilles tendon by inhibiting the Nr4a1/Wnt/β-catenin signaling pathway
Journal: Experimental Cell Research
DOI: 10.1016/j.yexcr.2025.114824
IF: 3.5
Application: WB,IHC
Reactivity: Mouse
Publish date: 2025 Nov
-
Nr4a1 enhances Wnt4 transcription to promote mesenchymal stem cell osteogenesis and alleviates inflammation-inhibited bone regeneration
Journal: Molecular Therapy
DOI:
IF: 12.4
Application: WB
Reactivity: Mouse
Publish date: 2024 Mar
-
COG1410 regulates microglial states and protects retinal ganglion cells in retinal ischemia-reperfusion injury
Journal: Experimental Eye Research
DOI:
IF: 3.4
Application: WB
Reactivity: Mouse
Publish date: 2023 Oct
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