Applications
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WB
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ICC/IF
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IHC-P
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IP
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FC
REACTIVITY
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Human
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Mouse
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Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
NLRP3 Recombinant Rabbit Monoclonal Antibody [SC06-23] (ET1610-93)
Immunogen
Recombinant protein within human nlrp3 aa 5-161 / 1036.
Host
Rabbit
Positive Control
Human lung tissue lysates, Hela, HUVEC, PMVEC, mouse bladder tissue, mouse spleen tissue, human lung carcinoma tissue, Jurkat, human colon carcinoma, human liver carcinoma.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SC06-23
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
118 kDa (Predicted band size)
Isotype
IgG
APPLICATION DILUTION
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WB
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1:500-1:1,000
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ICC/IF
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1:100-1:500
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IHC-P
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1:50-1:400
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FC
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1:50-1:100
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IP
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Use at an assay dependent concentration.
TARGET
UNIPROT #
PROTEIN NAME
NLRP3
SYNONYMS
AGTAVPRL antibody;AII/AVP antibody;Angiotensin/vasopressin receptor AII/AVP like antibody;Angiotensin/vasopressin receptor AII/AVP-like antibody;C1orf7 antibody;Caterpiller protein 1.1 antibody;CIAS 1 antibody;CIAS1 antibody;CLR1.1 antibody;Cold autoinflammatory syndrome 1 antibody;Cold autoinflammatory syndrome 1 protein antibody;Cryopyrin antibody;Familial cold autoinflammatory syndrome antibody;FCAS antibody;FCU antibody;LRR and PYD domains-containing protein 3 antibody;Muckle-Wells syndrome antibody;MWS antibody;NACHT antibody;NACHT LRR and PYD containing protein 3 antibody;NALP 3 antibody;NALP3 antibody;NALP3_HUMAN antibody;NLRP3 antibody;PYPAF 1 antibody;PYPAF1 antibody;PYRIN containing APAF1 like protein 1 antibody;PYRIN-containing APAF1-like protein 1 antibody
SEQUENCE SIMILARITIES
Belongs to the NLRP family.
TISSUE SPECIFICITY
Predominantly expressed in macrophages. Also expressed in dendritic cells, B- and T-cells (at protein level). Expressed in LPS-treated granulocytes, but not in resting cells (at protein level). Expression in monocytes is very weak (at protein level). Expressed in stratified non-keratinizing squamous epithelium, including oral, esophageal and ectocervical mucosa and in the Hassall's corpuscles in the thymus. Also, detected in the stratified epithelium covering the bladder and ureter (transitional mucosa) (at protein level). Expressed in lung epithelial cells (at protein level). Expressed in chondrocytes. Expressed at low levels in resting osteoblasts.
POST-TRANSLATIONAL MODIFICATION
The disulfide bond in the pyrin domain might play a role in reactive oxygen species-mediated activation.; Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Ubiquitination does not lead to degradation, but inhibits inflammasome activation (By similarity). Deubiquitination is catalyzed by BRCC3 and associated with NLRP3 activation and inflammasome assembly. This process can be induced by the activation of Toll-like receptors (by LPS), through a non-transcriptional pathway dependent on the mitochondrial production of reactive oxygen species, and by ATP.
SUBCELLULAR LOCATION
Cytoplasm, Inflammasome, Secreted, Nucleus, Endoplasmic reticulum.
FUNCTION
As the sensor component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of NLRP3, PYCARD and CASP1 (and possibly CASP4 and CASP5). Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu. Activation of NLRP3 inflammasome is also required for HMGB1 secretion. The active cytokines and HMGB1 stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death. Under resting conditions, NLRP3 is autoinhibited. NLRP3 activation stimuli include extracellular ATP, reactive oxygen species, K+ efflux, crystals of monosodium urate or cholesterol, amyloid-beta fibers, environmental or industrial particles and nanoparticles, cytosolic dsRNA, etc. However, it is unclear what constitutes the direct NLRP3 activator. Activation in presence of cytosolic dsRNA is mediated by DHX33. Independently of inflammasome activation, regulates the differentiation of T helper 2 (Th2) cells and has a role in Th2 cell-dependent asthma and tumor growth (By similarity). During Th2 differentiation, required for optimal IRF4 binding to IL4 promoter and for IRF4-dependent IL4 transcription. Binds to the consensus DNA sequence 5'-GRRGGNRGAG-3'. May also participate in the transcription of IL5, IL13, GATA3, CCR3, CCR4 and MAF.
CITATIONS
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Li, Feng et al.
AT-533, a Hsp90 inhibitor, attenuates HSV-1-induced inflammation. | Biochemical Pharmacology [2019]
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WB
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CO-IP
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BV2 cells
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Citation
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Wang, H., Huang, M., Wang, ...
Microglial TLR4-induced TAK1 phosphorylation and NLRP3 activation mediates neuroinflammation and contributes to chronic morphine-induced antinociceptive tolerance. Pharmacological research, 165, 105482.
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WB
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Mouse
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Citation
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Tao Xu;Aizhong Wang
Spinal TLR4/P2X7 Receptor-Dependent NLRP3 Inflammasome Activation Contributes to the Development of Tolerance to Morphine-Induced Antinociception
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WB
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mice
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Citation
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Yi, Xiaowei et al.
The effect of NLRP inflammasome on the regulation of AGEs-induced inflammatory response in human periodontal ligament cells. | Journal of Periodontal Research [2019]
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WB
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Human periodontal ligament cells
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Citation
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