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NINJ1/Ninjurin-1 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500367

NINJ1/Ninjurin-1 Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

NINJ1/Ninjurin-1 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within human Ninjurin-1 aa 1 – 80 (Extracellular).

Species Reactivity

Human, Mouse

Validated Applications

WB

Molecular Weight

Predicted band size: 16 kDa

Positive Control

Human liver tissue lysate, RAW264.7 cell lysate, Mouse kidney tissue lysate, Mouse liver tissue lysate, Mouse testis tissue lysate, Mouse skin tissue lysate.

Conjugation

unconjugated

RRID

AB_3071452

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Homophilic transmembrane adhesion molecule involved in various processes such as inflammation, cell death, axonal growth, cell chemotaxis and angiogenesis. Promotes cell adhesion by mediating homophilic interactions via its extracellular N-terminal adhesion motif (N-NAM). Involved in the progression of the inflammatory stress by promoting cell-to-cell interactions between immune cells and endothelial cells. Involved in leukocyte migration during inflammation by promoting transendothelial migration of macrophages via homotypic binding. Promotes the migration of monocytes across the brain endothelium to central nervous system inflammatory lesions. Acts as a regulator of Toll-like receptor 4 (TLR4) signaling triggered by lipopolysaccharide (LPS) during systemic inflammation; directly binds LPS. Acts as a mediator of both programmed and necrotic cell death. Plays a key role in the induction of plasma membrane rupture during programmed and necrotic cell death: oligomerizes in response to death stimuli to mediate plasma membrane rupture (cytolysis), leading to release intracellular molecules named damage-associated molecular patterns (DAMPs) that propagate the inflammatory response. Plays a role in nerve regeneration by promoting maturation of Schwann cells. Acts as a regulator of angiogenesis. Promotes the formation of new vessels by mediating the interaction between capillary pericyte cells and endothelial cells. Promotes osteoclasts development by enhancing the survival of prefusion osteoclasts. Also involved in striated muscle growth and differentiation.

Background References

1. Araki T., Milbrandt J. Ninjurin, a novel adhesion molecule, is induced by nerve injury and promotes axonal growth. Neuron 17:353-361(1996).

2. Shin M.W., Bae S.J., Wee H.J., Lee H.J., Ahn B.J., Le H., Lee E.J., Kim R.H., Lee H.S., Seo J.H., Park J.H., Kim K.W. Ninjurin1 regulates lipopolysaccharide-induced inflammation through direct binding. Int. J. Oncol. 48:821-828(2016).

Subcellular Location

Cell membrane, Secreted.

UNIPROT

SwissProt: Q92982 Human

SwissProt: O70131 Mouse

Synonyms

Nerve injury induced protein 1 antibody

Nerve injury-induced protein 1 antibody

NIN1 antibody

NINJ1 antibody

NINJ1_HUMAN antibody

NINJURIN antibody

Ninjurin-1 antibody

Images

  • Western blot analysis of NINJ1/Ninjurin-1 on different lysates with Rabbit anti-NINJ1/Ninjurin-1 antibody (<a href="/products/HA500367" style="font-weight: bold;text-decoration: underline;">HA500367</a>) at 1/1,000 dilution.<br /><br />Lane 1: Human liver tissue lysate (40 µg/Lane)<br />Lane 2: RAW264.7 cell lysate (20 µg/Lane)<br />Lane 3: Mouse kidney tissue lysate (40 µg/Lane)<br />Lane 4: Mouse liver tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 16 kDa<br />Observed band size: 16 kDa<br /><br />Exposure time: 1 minute 40 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA500367" style="font-weight: bold;text-decoration: underline;">HA500367</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of NINJ1/Ninjurin-1 on different lysates with Rabbit anti-NINJ1/Ninjurin-1 antibody (HA500367) at 1/1,000 dilution.

    Lane 1: Human liver tissue lysate (40 µg/Lane)
    Lane 2: RAW264.7 cell lysate (20 µg/Lane)
    Lane 3: Mouse kidney tissue lysate (40 µg/Lane)
    Lane 4: Mouse liver tissue lysate (40 µg/Lane)

    Predicted band size: 16 kDa
    Observed band size: 16 kDa

    Exposure time: 1 minute 40 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500367) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of NINJ1/Ninjurin-1 on different lysates with Rabbit anti-NINJ1/Ninjurin-1 antibody (<a href="/products/HA500367" style="font-weight: bold;text-decoration: underline;">HA500367</a>) at 1/1,000 dilution.<br /><br />Lane 1: RAW264.7 cell lysate<br />Lane 2: Mouse testis tissue lysate<br />Lane 3: Mouse kidney tissue lysate<br />Lane 4: Mouse skin tissue lysate<br />Lane 5: Mouse liver tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 16 kDa<br />Observed band size: 16 kDa<br /><br />Exposure time: 3 minute 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA500367" style="font-weight: bold;text-decoration: underline;">HA500367</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of NINJ1/Ninjurin-1 on different lysates with Rabbit anti-NINJ1/Ninjurin-1 antibody (HA500367) at 1/1,000 dilution.

    Lane 1: RAW264.7 cell lysate
    Lane 2: Mouse testis tissue lysate
    Lane 3: Mouse kidney tissue lysate
    Lane 4: Mouse skin tissue lysate
    Lane 5: Mouse liver tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 16 kDa
    Observed band size: 16 kDa

    Exposure time: 3 minute 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500367) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation