PRODUCT CODE: EM31105

Myc Tag Mouse Monoclonal Antibody [A3-B4] (EM31105)

Applications

  • WB

  • ICC

  • ELISA

  • IP

  • FC

  • IP

REACTIVITY

Western blot analysis of Myc Tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM31105, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: C-terminal Myc-tagged recombinant protein<br />
Lane 2: N-terminal Myc-tagged recombinant protein
  • Western blot analysis of Myc Tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM31105, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: C-terminal Myc-tagged recombinant protein<br />
Lane 2: N-terminal Myc-tagged recombinant protein
  • ICC staining of Myc Tag in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM31105, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Myc tag was immunoprecipitated in 2µg C terminal Myc Tag fusion protein lysate with EM31105 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using R1208-1 at 1/1,000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 60 mins at room temperature.<br />
<br />
Lane 1: Myc Tag fusion protein lysate (input).<br />
Lane 2: EM1902-38 IP in Myc Tag fusion protein lysate.<br />
Lane 3: EM31105 IP in Myc Tag fusion protein lysate.<br />
Lane 4: Mouse IgG instead of EM31105 in Myc Tag fusion protein lysate.<br />
<br />
Blocking/Dilution buffer: 5% NFDM/TBST
  • Myc tag was immunoprecipitated in 2µg N terminal Myc Tag fusion protein lysate with EM31105 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using R1208-1 at 1/1,000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 60 mins at room temperature.<br />
<br />
Lane 1: Myc Tag fusion protein lysate (input).<br />
Lane 2: EM1902-38 IP in Myc Tag fusion protein lysate.<br />
Lane 3: EM31105 IP in Myc Tag fusion protein lysate.<br />
Lane 4: Mouse IgG instead of EM31105 in Myc Tag fusion protein lysate.<br />
<br />
Blocking/Dilution buffer: 5% NFDM/TBST
Western blot analysis of Myc Tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM31105, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: C-terminal Myc-tagged recombinant protein
Lane 2: N-terminal Myc-tagged recombinant protein

Applications

  • WB

  • ICC

  • ELISA

  • IP

  • FC

  • IP

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Myc Tag Mouse Monoclonal Antibody [A3-B4] (EM31105)

Immunogen

Synthetic peptide immune sequence is eqkliseedlc-klh.

Host

Mouse

Positive Control

Myc-tagged recombinant protein.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A3-B4

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G purified.

Isotype

IgG2b

APPLICATION DILUTION

  • WB

  • 1:2,000

  • IP

  • 2-5 µg/ml.

TARGET

PROTEIN NAME

Myc Tag

SYNONYMS

avian myelocytomatosis viral oncogene homolog antibody; bHLHe39 antibody; c-Myc antibody; class E basic helix-loop-helix protein 39 antibody; MRTL antibody; MYC antibody; Myc Epitope Tag antibody; myc proto-oncogene protein antibody; myc-related translation/localization regulatory factor antibody; oncogene c-Myc antibody; proto-oncogene c-Myc antibody; protooncogene homologous to myelocytomatosis virus antibody; transcription factor p64 antibody; v-myc avian myelocytomatosis viral oncogene homolog antibody; v-myc myelocytomatosis viral oncogene homolog (avian) antibody

FUNCTION

Myc gene encodes for a transcription factor that is believed to regulate expression of 15% of all genes through binding on Enhancer Box sequences (E-boxes) and recruiting histone acetyltransferases (HATs). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. This Myc-Tag antibody detects Myc-tagged fusion proteins.

CITATIONS

  • Guo, Chuansheng et al.

    Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages. | Immunity [2018]

  • Zheng, Z., Zhang, X., Huang...

    Site-1 protease controls osteoclastogenesis by mediating LC3 transcription. Cell death and differentiation, 28(6), 2001–2018.