Positive control:
Lane 1: C-terminal Myc-tagged recombinant protein
Lane 2: N-terminal Myc-tagged recombinant protein
Applications
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WB
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ICC
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ELISA
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FC
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IP
SPECIFICATIONS
Product Type
Mouse monoclonal primary
Product Name
Myc Tag Mouse Monoclonal Antibody [A3-B4] (EM31105)
Immunogen
Synthetic peptide immune sequence is eqkliseedlc-klh.
Host
Mouse
Positive Control
Myc-tagged recombinant protein.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A3-B4
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 ug/ul
PURIFICATION
Protein G affinity purified.
Isotype
IgG2b
APPLICATION DILUTION
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WB
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1:2,000
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IP
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2-5 µg/ml.
TARGET
PROTEIN NAME
Myc Tag
SYNONYMS
avian myelocytomatosis viral oncogene homolog antibody; bHLHe39 antibody; c-Myc antibody; class E basic helix-loop-helix protein 39 antibody; MRTL antibody; MYC antibody; Myc Epitope Tag antibody; myc proto-oncogene protein antibody; myc-related translation/localization regulatory factor antibody; oncogene c-Myc antibody; proto-oncogene c-Myc antibody; protooncogene homologous to myelocytomatosis virus antibody; transcription factor p64 antibody; v-myc avian myelocytomatosis viral oncogene homolog antibody; v-myc myelocytomatosis viral oncogene homolog (avian) antibody
FUNCTION
Myc gene encodes for a transcription factor that is believed to regulate expression of 15% of all genes through binding on Enhancer Box sequences (E-boxes) and recruiting histone acetyltransferases (HATs). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. This Myc-Tag antibody detects Myc-tagged fusion proteins.
CITATIONS
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Zheng, Z., Zhang, X., Huang...
Site-1 protease controls osteoclastogenesis by mediating LC3 transcription. Cell death and differentiation, 28(6), 2001–2018.
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WB
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IF
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IP
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Mouse
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Citation
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Guo, Chuansheng et al.
Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages. | Immunity [2018]
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WB
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None
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Citation
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