mTOR Recombinant Rabbit Monoclonal Antibody [SU30-00] (ET1608-5)
Catalog# ET1608-5
Rabbit Monoclonal to mTOR
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WB
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IHC-P
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IP
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IF-Cell
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: MCF7 cell lysate (20 µg/Lane)
Lane 3: HEK-293 cell lysate (20 µg/Lane)
Lane 4: HepG2 cell lysate (20 µg/Lane)
Lane 5: C2C12 cell lysate (20 µg/Lane)
Lane 6: PC-12 cell lysate (20 µg/Lane)
Lane 7: C6 cell lysate (20 µg/Lane)
Lane 8: Mouse testis tissue lysate (20 µg/Lane)
Lane 9: Rat testis tissue lysate (20 µg/Lane)
Predicted band size: 289 kDa
Observed band size: 289 kDa
Exposure time: 24 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-5) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Safety datasheet
Select your chosen country/region
- MSDS_HUABIO.pdf
- MSDS_HUABIO.pdf
- MSDS_ET1608-5_Europe.pdf
- No MSDS Found
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
mTOR Recombinant Rabbit Monoclonal Antibody [SU30-00] (ET1608-5)
Immunogen
Synthetic peptide within human mtor aa 2400-2440.
Host
Rabbit
Positive Control
HeLa cell lysate, MCF7 cell lysate, HEK-293 cell lysate, HepG2 cell lysate, C2C12 cell lysate, PC-12 cell lysate, C6 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, rat heart tissue lysate, SiHa cell lysate, human breast carcinoma tissue, HeLa, NIH/3T3, human kidney tissue, mouse testis tissue, mouse kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SU30-00
RRID
APPLICATION DILUTION
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WB
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1:5,000
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IHC-P
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1:200-1:1,000
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IF-cell
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1:50-1:200
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 289 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
mTOR
SYNONYMS
dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) antibody;FK506 binding protein 12 rapamycin associated protein 1 antibody;FK506 binding protein 12 rapamycin associated protein 2 antibody;FK506 binding protein 12 rapamycin complex associated protein 1 antibody;FK506-binding protein 12-rapamycin complex-associated protein 1 antibody;FKBP rapamycin associated protein antibody;FKBP12 rapamycin complex associated protein antibody;FKBP12-rapamycin complex-associated protein 1 antibody;FKBP12-rapamycin complex-associated protein antibody;FLJ44809 antibody;FRAP antibody;FRAP1 antibody;FRAP2 antibody;Mammalian target of rapamycin antibody;Mechanistic target of rapamycin antibody;mTOR antibody;MTOR_HUMAN antibody;OTTHUMP00000001983 antibody;RAFT1 antibody;Rapamycin and FKBP12 target 1 antibody;Rapamycin associated protein FRAP2 antibody;Rapamycin target protein 1 antibody;Rapamycin target protein antibody;RAPT1 antibody;Serine/threonine-protein kinase mTOR antibody
SEQUENCE SIMILARITIES
Belongs to the PI3/PI4-kinase family.
TISSUE SPECIFICITY
Expressed in numerous tissues, with highest levels in testis.
POST-TRANSLATIONAL MODIFICATION
Autophosphorylates when part of mTORC1 or mTORC2. Phosphorylation at Ser-1261, Ser-2159 and Thr-2164 promotes autophosphorylation. Phosphorylation in the kinase domain modulates the interactions of MTOR with RPTOR and PRAS40 and leads to increased intrinsic mTORC1 kinase activity. Phosphorylation at Thr-2173 in the ATP-binding region by AKT1 strongly reduces kinase activity.
SUBCELLULAR LOCATION
Endoplasmic reticulum membrane, Microsome membrane, PML body, Golgi apparatus membrane, Cytoplasm, Lysosome, Lysosome membrane, Mitochondrion outer membrane, phagosome.
FUNCTION
The mammalian target of rapamycin (mTOR), also referred to as the mechanistic target of rapamycin, and sometimes called FK506-binding protein 12-rapamycin-associated protein 1 (FRAP1), is a kinase that in humans is encoded by the MTOR gene. mTOR is a member of the phosphatidylinositol 3-kinase-related kinase family of protein kinases. mTOR links with other proteins and serves as a core component of two distinct protein complexes, mTOR complex 1 and mTOR complex 2, which regulate different cellular processes. In particular, as a core component of both complexes, mTOR functions as a serine/threonine protein kinase that regulates cell growth, cell proliferation, cell motility, cell survival, protein synthesis, autophagy, and transcription. As a core component of mTORC2, mTOR also functions as a tyrosine protein kinase that promotes the activation of insulin receptors and insulin-like growth factor 1 receptors. mTORC2 has also been implicated in the control and maintenance of the actin cytoskeleton.
CITATIONS
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Liu Xinying,et al
Genipin promotes the apoptosis and autophagy of neuroblastoma cells by suppressing the PI3K/AKT/mTOR pathway
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WB
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Human
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Citation
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Huang Z , Dai L, Peng F,et al
mTOR signaling pathway regulates embryonic development and rapid growth of triploid crucian carp
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WB
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Zebrafish
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Citation
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Chen, X., Wang, J., Song, L., Yu, Y., Shi, M., Jiang, W., Liu, X., & He, X.
Downregulation of fibulin-4 inhibits autophagy and promotes the sensitivity of esophageal squamous cell carcinoma cells to apatinib by activating the Akt-mTOR signaling pathway
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WB
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Human
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Citation
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Jing Xia, Qian long Dai, Siyue He, Hui-jie Jia, Xian-Guo Liu, Hui Hua, Min Zhou, Xiaobo Wang
Artesunate alleviates 5-fluorouracil-induced intestinal damage by suppressing cellular senescence and enhances its antitumor activity
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WB
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Mouse
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Citation
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Li Jingyang,et al
Baicalein is a potential agent targeting glutamine that induces apoptosis by inhibiting the glutamine-mTOR metabolic pathway in lung cancer
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WB
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Human
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Citation
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Zhiruo Wang ,et al
Zotarolimus alleviates post-trabeculectomy fibrosis via dual functions of anti-inflammation and regulating AMPK/mTOR axis
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IF
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Citation
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Inhibition of STX17–SNAP29–VAMP8 complex formation by costunolide sensitizes ovarian cancer cells to cisplatin via the AMPK/mTOR signaling pathway
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WB
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Human
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Citation
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Li PIng,et al
Leptin protects chondrocytes by inhibiting autophagy via phosphoinositide 3 kinase/protein kinase B/mammalian target of rapamycin signaling pathway
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WB
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Rat
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Citation
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Ji, X., Du, W., Che, W., Wang, L., & Zhao, L.
Apigenin Inhibits the Progression of Osteoarthritis by Mediating Macrophage Polarization
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WB
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Mouse
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Citation
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Tang, Y., Jin, Y. H., Li, H. L., Xin, H., Chen, J. D., Li, X. Y., & Pan, Y. F.
PBRM1 deficiency oncogenic addiction is associated with activated AKT–mTOR signalling and aerobic glycolysis in clear cell renal cell carcinoma cells
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WB
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Human
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Citation
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Hu Jiahe,et al
Engineered-Doping Strategy for Self-Sufficient Reactive Oxygen Species Blossom to Amplify Ferroptosis/Cuproptosis Sensibilization in Hepatocellular Carcinoma Treatment
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WB
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Human
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Citation
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Yao Peixue,et al
IL-17A regulates collagen synthesis after autophagy in bronchial fibroblasts through the PI3K/AKT/mTOR pathway
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WB
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Rat
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Citation
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Shusen Zheng
Combination of HSP90 and autophagy inhibitors promotes hepatocellular carcinoma apoptosis following incomplete thermal ablation
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WB
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Mouse
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Citation
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Koda, S., Zhang, B., Zhou, Q. Y., Xu, N., Li, J., Liu, J. X., Liu, M., Lv, Z. Y., Wang, J. L., Shi, Y., Gao, S., Yu, Q., Li, X. Y., Xu, Y. H., Chen, J. X., Tekengne, B., Adzika, G. K., Tang, R. X., Sun, H., Zheng, K. Y., … Yan, C.
β2-Adrenergic Receptor Enhances the Alternatively Activated Macrophages and Promotes Biliary Injuries Caused by Helminth Infection
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WB
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Mouse
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Citation
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Icariin alleviates osteoarthritis through PI3K/Akt/mTOR/ULK1 signaling pathway
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WB
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Human
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Citation
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Rao Zichen
HNRNP I promotes IRAK1 degradation to reduce podocyte apoptosis and inflammatory response alleviating renal injury in diabetic nephropathy
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WB
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Human
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Citation
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Hui Li,et al
Targeting NMDAR2A: Esketamine's Impact on BV2 Microglial Pro-inflammatory Polarization
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WB
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Mouse
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Citation
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Gomisin N attenuated cerebral ischemia-reperfusion injury through inhibition of autophagy by activating the PI3K/AKT/mTOR pathway
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WB
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Mouse
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Citation
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Curcumin protects human umbilical vein endothelial cells against high oxidized low density lipoprotein-induced lipotoxicity and modulates autophagy
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WB
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Human
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Citation
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A novel and potent dihydroorotate dehydrogenase inhibitor suppresses the proliferation of colorectal cancer by inducing mitochondrial dysfunction and DNA damage
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WB
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Human
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Citation
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Chen, Y., Lu, Z., Qi, C., Yu, C., Li, Y., Huan, W., Wang, R., Luo, W., Shen, D., Ding, L., Ren, L., Xie, H., Xue, D., Wang, M., Ni, K., Xia, L., Qian, J., & Li, G.
N6-methyladenosine-modified TRAF1 promotes sunitinib resistance by regulating apoptosis and angiogenesis in a METTL14-dependent manner in renal cell carcinoma
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WB
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Human
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Citation
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Li L, Li S, Pan Z, Zhang Y, Hua Z
Bilirubin impacts microglial autophagy via the Akt–mTOR signaling pathway
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WB
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Rat
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Citation
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CD147 Mediates 5-Fluorouracil Resistance in Colorectal Cancer by Reprogramming Glycolipid Metabolism
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WB
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Human
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Citation
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Mettl14 sustains FOXP3 expression to promote the differentiation and functions of induced-regulatory T cells via the mTOR signaling pathway
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WB
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Mouse
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Citation
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Yangfan Liu , Yu Sun , Jin Yang ,et al
DNMT1-targeting remodeling global DNA hypomethylation for enhanced tumor suppression and circumvented toxicity in oral squamous cell carcinoma
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WB
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Human
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Citation
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Yun, X., Fang, Y., Lv, C., Qiao, S., Tao, Y., Dai, Y., & Xia, Y.
Inhibition of the activation of γδT17 cells throughPPARγ–PTEN/Akt/GSK3β/NFAT pathwaycontributes to the anti-colitis effect of madecassicacid
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WB
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Mouse
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Citation
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Assessment of the anti-inflammatory mechanism of quercetin 3,7-dirhamnoside using an integrated pharmacology strategy
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WB
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Mouse
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Citation
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Xia Yu,et al
Artesunate-loaded solid lipid nanoparticles resist esophageal squamous cell carcinoma by inducing Ferroptosis through inhibiting the AKT/mTOR signaling
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WB
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Mouse
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Citation
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Chen, M., Jing, D., Ye, R., Yi, J., & Zhao, Z.
PPARβ/δ accelerates bone regeneration in diabetic mellitus by enhancing AMPK/mTOR pathway-mediated autophagy
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WB
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Rat
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Citation
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Lu-Sha Cen,et al
Shikonin protects mitochondria through the NFAT5/AMPK pathway for the treatment of diabetic wounds
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WB
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Human
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Citation
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Dissecting the novel abilities of aripiprazole: The generation of anti-colorectal cancer effects by targeting Gαq via HTR2B
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WB
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Mouse
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Citation
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Carvedilol exhibits anti-acute T lymphoblastic leukemia effect in vitro and in vivo via inhibiting β-ARs signaling pathway
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WB
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Human
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Citation
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