Positive control:
Lane 1: Hela
Lane 2: Mouse kidney tissue
Lane 3: K562
Applications
-
WB
-
IHC-P
-
ICC
REACTIVITY
-
Human
-
Mouse
SPECIFICATIONS
Product Type
Mouse monoclonal primary
Product Name
MTERFD1 Mouse Monoclonal Antibody [A8-A10] (EM1701-29)
Immunogen
Recombinant protein with human mterfd1 aa 29-417 / 417.
Host
Mouse
Positive Control
Hela, mouse kidney tissue lysate, K562, human liver tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A8-A10
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
48/38 kDa
Isotype
IgG1
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
-
IHC-P
-
1:50-1:200
-
ICC
-
1:50
TARGET
UNIPROT #
PROTEIN NAME
MTERFD1
SYNONYMS
CGI 12 antibody; mitochondrial antibody; Mitochondrial transcription termination factor 3 antibody; MTER1_HUMAN antibody; MTERF domain containing 1 antibody; mTERF domain containing protein 1 mitochondrial antibody; mTERF domain-containing protein 1 antibody; mTERF3 antibody; Mterfd1 antibody; Transcription termination factor 3, mitochondrial antibody
SEQUENCE SIMILARITIES
Belongs to the mTERF family.
TISSUE SPECIFICITY
Highly expressed in heart, liver, kidney and testis. Detected at lower levels in brain, spleen and lung.
SUBCELLULAR LOCATION
Mitochondrion.
FUNCTION
MTERFD1 belongs to the MTERF family which consists of four members including MTERF1, MTERF2, MTERF3 and MTERF4. Previous research showed that MTERFD1 is a mitochondrial protein that interacts with the mitochondrial DNA promoter region and decreases transcription initiation in mammalian mitochondria. This negative regulation is likely important for fine-tuning mitochondria transcription in response to physiological demands. Human mitochondrial transcription termination factor domain 1 (MTERFD1) was mutated in many types of cancer. In vivo and in vitro data indicated that MTERFD1 gene possesses oncogenic properties.