MTERFD1 Mouse Monoclonal Antibody [A8-A10] (EM1701-29)
Catalog# EM1701-29
Mouse Monoclonal to MTERFD1
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WB
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IHC-P
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IF-Cell
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Human
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: K-562 cell lysate
Lane 4: HEK-293 cell lysate
Lane 5: SH-SY5Y cell lysate
Lane 6: SK-OV-3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 40 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-29) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
MTERFD1 Mouse Monoclonal Antibody [A8-A10] (EM1701-29)
Immunogen
Recombinant protein with human mterfd1 aa 29-417 / 417.
Host
Mouse
Positive Control
HeLa cell lysate, HepG2 cell lysate, K-562 cell lysate, HEK-293 cell lysate, SH-SY5Y cell lysate, SK-OV-3 cell lysate, human liver tissue, A431.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A8-A10
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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IHC-P
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1:50-1:200
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IF-Cell
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1:50
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 48 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
MTERFD1
SYNONYMS
CGI 12 antibody;mitochondrial antibody;Mitochondrial transcription termination factor 3 antibody;MTER1_HUMAN antibody;MTERF domain containing 1 antibody;mTERF domain containing protein 1 mitochondrial antibody;mTERF domain-containing protein 1 antibody;mTERF3 antibody;Mterfd1 antibody;Transcription termination factor 3, mitochondrial antibody
SEQUENCE SIMILARITIES
Belongs to the mTERF family.
TISSUE SPECIFICITY
Highly expressed in heart, liver, kidney and testis. Detected at lower levels in brain, spleen and lung.
SUBCELLULAR LOCATION
Mitochondrion.
FUNCTION
MTERFD1 belongs to the MTERF family which consists of four members including MTERF1, MTERF2, MTERF3 and MTERF4. Previous research showed that MTERFD1 is a mitochondrial protein that interacts with the mitochondrial DNA promoter region and decreases transcription initiation in mammalian mitochondria. This negative regulation is likely important for fine-tuning mitochondria transcription in response to physiological demands. Human mitochondrial transcription termination factor domain 1 (MTERFD1) was mutated in many types of cancer. In vivo and in vitro data indicated that MTERFD1 gene possesses oncogenic properties.
CITATIONS
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Sun Meitao,et al
Prognostic Role of Mitochondrial Transcription Termination Factor 3 in Thyroid Carcinoma
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IHC
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Human
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Citation
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