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Mouse LIF, C-His Tag Protein (Active) (HA210735)

Price: Regular price $249.00

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Catalog# HA210735-50UG

Biologically active: yes

Reacts with

  • Mouse

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Product Details

Product Type

Protein

Product Name

Mouse LIF, C-His Tag Protein (Active) (HA210735)

Predicted molecular mass

21.2 kD

Protein construction description

A DNA sequence encoding the mouse LIF protein (P09056) (Ser 24-Phe 203) was expressed with a His tag at the C-terminus.

Accession

P09056

Protein construction

LIF (24-203) | His

Source

HEK293

Bio Activity

Mouse LIF can replace fibroblast feeding layer or other conditioned medium under the condition of 1000 U/mL, maintain the undifferentiated state of mouse embryonic stem cells ES.W4 and promote cell proliferation.

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.

Species

Mouse

Background

The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.

Usage Guide

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.

Storage

Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20C to -80C It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.

Reconstitution

Reconstitute at 250 μg/ml in sterile water.

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