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Mammaglobin A Recombinant Mouse Monoclonal Antibody [A9A2-R]

Usd: 350

Specification

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Catalog# HA601136

Mammaglobin A Recombinant Mouse Monoclonal Antibody [A9A2-R]

Application

  • IHC-P

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Mammaglobin A Recombinant Mouse Monoclonal Antibody [A9A2-R]

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Synthetic peptide within Human Mammaglobin A aa 21-70 / 93.

Species Reactivity

Human

Validated Applications

IHC-P

Molecular Weight

Predicted band size: 10 kDa

Positive Control

Human skin tissue, human breast tissue.

Conjugation

unconjugated

Clone Number

A9A2-R

RRID

AB_3071858

Reactivity Data

IHC-P
Human
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:2,000

Target

Function

Mammaglobin-A also known as secretoglobin family 2A member 2 is a protein that in humans is encoded by the SCGB2A2 gene. SCGB2A2 is a member of the superfamily of secretoglobins, a group of small dimeric secreted and sometimes glycosylated proteins. Expressed mainly in mucosa, secretoglobins seem to be involved in cell signalling, immune response, and chemotaxis, and may also serve as transporters for steroid hormones in humans. SCGB2A2 expression is highly specific of mammary tissue, and is increasingly used for identification and detection of disseminated breast cancer cells. Mammaglobin is breast-associated glycoprotein distantly related to secretoglobin family that includes human uteroglobin and lipophilin. Anti-mammaglobin labels cytoplasm of normal breast epithelial cells as well as primary and metastatic breast carcinomas. Absence of mammaglobin expression is typically seen in prostate, kidney, colon, rectum, small intestine, stomach, pancreas, lung and thyroid tissue. Mammaglobin may be used as part of an immunohistochemical panel for determination of metastatic breast carcinoma and tumor of unknown primary origin.

Background References

1. Liu Z et al. Identification and characterization of mammaglobin-A epitope in heterogenous breast cancers for enhancing tumor-targeting therapy. Signal Transduct Target Ther. 2020 May

2. Talaat IM et al. Bone marrow mammaglobin-1 (SCGB2A2) immunohistochemistry expression as a breast cancer specific marker for early detection of bone marrow micrometastases. Sci Rep. 2020 Aug

Subcellular Location

Secreted.

UNIPROT

SwissProt: Q13296 Human

Synonyms

Mammaglobin 1 antibody

Mammaglobin-1 antibody

Mammaglobin-A antibody

MGB1 antibody

Scgb2a2 antibody

Secretoglobin family 2A member 2 antibody

SG2A2 antibody

SG2A2_HUMAN antibody

UGB2 antibody

Images

  • Immunohistochemical analysis of paraffin-embedded human skin tissue with Mouse anti-Mammaglobin A antibody (<a href="/products/HA601136" style="font-weight: bold;text-decoration: underline;">HA601136</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601136" style="font-weight: bold;text-decoration: underline;">HA601136</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human skin tissue with Mouse anti-Mammaglobin A antibody (HA601136) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601136) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-Mammaglobin A antibody (<a href="/products/HA601136" style="font-weight: bold;text-decoration: underline;">HA601136</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601136" style="font-weight: bold;text-decoration: underline;">HA601136</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-Mammaglobin A antibody (HA601136) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601136) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded human kidney (Negative) tissue with Mouse anti-Mammaglobin A antibody (<a href="/products/HA601136" style="font-weight: bold;text-decoration: underline;">HA601136</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601136" style="font-weight: bold;text-decoration: underline;">HA601136</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded human kidney (Negative) tissue with Mouse anti-Mammaglobin A antibody (HA601136) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601136) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"