mAID tag Rabbit Polyclonal Antibody
Catalog# ER1802-93
mAID tag Rabbit Polyclonal Antibody
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WB
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Species independent
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unconjugated
Overview
Product Name
mAID tag Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide immune sequence is PKDPAKPPAKAQVVGWPC.
Species Reactivity
Species independent
Validated Applications
WB
Positive Control
Mini-AID-tagged fusion protein.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:1,000-1:5,000
Target
Function
Auxin-inducible degron (AID) technology allows rapid depletion of proteins in animal cells and fungi. Auxin includes chemicals such as IAA (indole-3-aceticacid) and NAA (1-naphtalene acetic acid) and includes inhibitors in plants. For the degradation, and SCF ubiquitin ligase complex containing the TIR1 protein is activated via the association of auxin with TIR1. Because the core SCF components are conserved in all eukaryotic cells, it is possible to transplant the degradation pathway to yeast and mammalian cells by expressing TIR1. In those cells expressing TIR1, a protein fused with degron (AID degron) derived from AUX/IAA can be rapidly degraded in the presence of auxin. Anti-mini-AID tag can detected both mini-AID-tag and full-length AID-tag.
Background References
1. Clift D et al. A Method for the Acute and Rapid Degradation of Endogenous Proteins. Cell 171(7):1692-1706 (2017).
Images
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Western blot analysis of Mini-AID-tag on fusion protein using anti-Mini-AID-tag antibody at 1/5,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"