Positive control:
Lane 1: mouse liver tissue lysate
Lane 2: mouse lung tissue lysate
Lane 3: human liver tissue lysate
Lane 4: human lung tissue lysate
Applications
-
WB
-
ICC/IF
-
IHC-P
-
IP
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
LRP1 Recombinant Rabbit Monoclonal Antibody [SA0290] (ET1601-1)
Immunogen
Synthetic peptide within human lrp1 aa 4,471-4,520 / 4,544.
Host
Rabbit
Positive Control
Mouse liver tissue lysate, mouse lung tissue lysate, human liver tissue lysate, human lung tissue lysate, Hela, MCF-7, HUVEC, human lung tissue, mouse brain tissue, mouse liver tissue, human liver tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SA0290
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
85 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:5,000
-
ICC/IF
-
1:50-1:200
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
-
IP
-
Use at an assay dependent concentration.
TARGET
UNIPROT #
PROTEIN NAME
LRP1
SYNONYMS
A2MR antibody; Alpha 2 macroglobulin receptor antibody; alpha 2MR antibody; Alpha-2-macroglobulin receptor antibody; APOER antibody; Apolipoprotein E receptor antibody; APR antibody; CD 91 antibody; CD91 antibody; CD91 antigen antibody; IGFBP3R antibody; LDL receptor related protein 1 antibody; Low density lipoprotein receptor related protein 1 antibody; Low density lipoprotein related protein 1 antibody; Low-density lipoprotein receptor-related protein 1 intracellular domain antibody; LRP 1 antibody; LRP 515 antibody; LRP 85 antibody; LRP antibody; LRP ICD antibody; LRP-1 antibody; LRP-515 antibody; LRP-85 antibody; Lrp1 antibody; LRP1 protein antibody; LRP1_HUMAN antibody; LRP1A antibody; LRP515 antibody; LRP85 antibody; LRPICD antibody; MGC88725 antibody; Prolow density lipoprotein receptor related protein 1 antibody; TbetaR V/LRP 1/IGFBP 3 receptor antibody; TbetaRV/LRP1/IGFBP3 receptor antibody; TGFBR 5 antibody; TGFBR5 antibody; Type V tgf beta receptor antibody; Low-density lipoprotein receptor-related protein 1 85 kDa subunit
SEQUENCE SIMILARITIES
Belongs to the LDLR family.
TISSUE SPECIFICITY
Most abundant in liver, brain and lung.
POST-TRANSLATIONAL MODIFICATION
Cleaved into a 85 kDa membrane-spanning subunit (LRP-85) and a 515 kDa large extracellular domain (LRP-515) that remains non-covalently associated. Gamma-secretase-dependent cleavage of LRP-85 releases the intracellular domain from the membrane.; The N-terminus is blocked.; Phosphorylated on serine and threonine residues.; Phosphorylated on tyrosine residues upon stimulation with PDGF. Tyrosine phosphorylation promotes interaction with SHC1.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus, Membrane.
FUNCTION
Members of the LDL receptor gene family, including LDLR (low density lipoprotein receptor), LRP1 (low density lipoprotein related protein), Megalin (also designated GP330), VLDLR (very low density lipoprotein receptor) and ApoER2 are characterized by a cluster of cysteine-rich class A repeats, epidermal growth factor (EGF)-like repeats, YWTD repeats and an O-linked sugar domain. LRP1, also designated LRP and ?-2-Macroglobulin receptor, is an endocytic receptor that mediates the uptake of at least 15 ligands, including ?-2-Macroglobulin and apoE. LRP1 is cleaved into a membrane subunit and an extracellular subunit, which remain non-covalently associated. Proper folding and trafficking of LRP1 is facilitated by the receptor-associated protein (RAP), a molecular chaperone. The uptake of all known ligands through LRP1 can be blocked by RAP, which induces a conformational change in the receptor that renders it unable to bind ligands. LRP1, which is expressed in brain, liver and lung, is also implicated in Alzheimer’s disease (AD), as the human LRP gene localizes to a potential AD locus on chromosome 12.