PRODUCT CODE: ER1802-21

KDM5B Rabbit Polyclonal Antibody (ER1802-21)

Applications

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

ICC staining KDM5B in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining KDM5B in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining KDM5B in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining KDM5B in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-KDM5B antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-KDM5B antibody. Counter stained with hematoxylin.
ICC staining KDM5B in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

KDM5B Rabbit Polyclonal Antibody (ER1802-21)

Immunogen

Synthetic peptide within human kdm5b aa 960-1,009 / 1,544.

Host

Rabbit

Positive Control

MCF-7, PC-3M, SH-SY5Y, rat epididymis tissue, mouse testis tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

179 kDa

Isotype

IgG

APPLICATION DILUTION

  • ICC

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

KDM5B

SYNONYMS

Cancer/testis antigen 31 antibody; CT31 antibody; Histone demethylase JARID1B antibody; JARID1B antibody; Jumonji AT rich interactive domain 1B antibody; Jumonji/ARID domain-containing protein 1B antibody; Kdm5b antibody; KDM5B_HUMAN antibody; Lysine (K) specific demethylase 5B antibody; Lysine demethylase 5B antibody; Lysine specific demethylase 5B antibody; Lysine-specific demethylase 5B antibody; PLU-1 antibody; PLU1 antibody; PPP1R98 antibody; Protein phosphatase 1 regulatory subunit 98 antibody; PUT1 antibody; putative DNA/chromatin binding motif antibody; RBBP2H1A antibody; RBP2 like antibody; RBP2-H1 antibody; Retinoblastoma-binding protein 2 homolog 1 antibody; Retinoblastoma-binding protein 2 homolog 1A antibody

SEQUENCE SIMILARITIES

Belongs to the JARID1 histone demethylase family.

TISSUE SPECIFICITY

Ubiquitously expressed, with highest levels in testis. Down-regulated in melanoma and glioblastoma. Up-regulated in breast cancer (at protein level).

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

PLU-1 is a large (1,544 amino acid) nuclear protein that interacts with brain factor-1 (BF-1) and paired box 9 (PAX9), both of which are developmental transcription factors. PLU-1 belongs to the testis-cancer antigen group of proteins and is a member of the ARID family of DNA binding proteins. It is a multi-domain protein with strong transcriptional repression properties. PLU-1 shows restricted expression in adult tissues, with high expression in testis and transient expression in the pregnant mammary gland. Both the PLU-1 gene and the PLU-1 protein product are specifically upregulated in breast cancer. PLU-1 may be important in meiotic transcription because of its apparent association with chromatin.