Interferon gamma Recombinant Rabbit Monoclonal Antibody [JM10-10] (ET1703-17)
Catalog# ET1703-17
Rabbit Monoclonal to Interferon gamma
-
WB
-
FC
-
Human
Lane 1: Human IFN-gamma recombinant protein, 50ng/Lane
Lane 2: Mouse IFN-gamma recombinant protein, 50ng/Lane
Lane 3: Rat IFN-gamma recombinant protein, 50ng/Lane
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-17) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Interferon gamma Recombinant Rabbit Monoclonal Antibody [JM10-10] (ET1703-17)
Immunogen
Synthetic peptide within n-terminal human interferon gamma.
Host
Rabbit
Positive Control
Human IFN-gamma recombinant protein, Jurkat.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM10-10
RRID
APPLICATION DILUTION
-
WB
-
1:1,000
-
FC
-
1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 19 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Interferon gamma
SYNONYMS
IFG antibody;IFI antibody;IFN gamma antibody;IFN, immune antibody;IFN-gamma antibody;IFNG antibody;IFNG_HUMAN antibody;Immune interferon antibody;Interferon gamma antibody
SEQUENCE SIMILARITIES
Belongs to the type II (or gamma) interferon family.
TISSUE SPECIFICITY
Released primarily from activated T lymphocytes.
POST-TRANSLATIONAL MODIFICATION
Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
SUBCELLULAR LOCATION
Secreted.
FUNCTION
Interferon gamma (IFN-γ) is a dimerized soluble cytokine that is the only member of the type II class of interferons. In humans, the IFN-γ protein is encoded by the IFNG gene. IFN-γ, or type II interferon, is a cytokine that is critical for innate and adaptive immunity against viral, some bacterial and protozoan infections. IFN-γ is an important activator of macrophages and inducer of major histocompatibility complex class II molecule expression. Aberrant IFN-γ expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN-γ in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFN-γ is produced predominantly by natural killer cells (NK) and natural killer T cells (NKT) as part of the innate immune response, and by CD4 Th1 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops as part of the adaptive immune response. IFN-γ is also produced by non-cytotoxic innate lymphoid cells (ILC), a family of immune cells first discovered in the early 2010s.
