PRODUCT CODE: R1510-3

IGF2R/M6PR Rabbit Polyclonal Antibody (R1510-3)

Applications

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

Immunocytochemical staining of Hela cells using anti-M6PR rabbit polyclonal antibody.
  • Immunocytochemical staining of Hela cells using anti-M6PR rabbit polyclonal antibody.
  • Immunocytochemical staining of SW480 cells using anti-M6PR rabbit polyclonal antibody.
  • Immunocytochemical staining of MCF-7 cells using anti-M6PR rabbit polyclonal antibody.
  • Immunohistochemical analysis of paraffin- embedded human tonsil tissue using anti-M6PR rabbit polyclonal antibody.
  • Immunohistochemical analysis of paraffin- embedded human lung carcinoma tissue using anti-M6PR rabbit polyclonal antibody.
  • Flow cytometric analysis of Hela cells with M6PR antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody.
Immunocytochemical staining of Hela cells using anti-M6PR rabbit polyclonal antibody.

Applications

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

IGF2R/M6PR Rabbit Polyclonal Antibody (R1510-3)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, SW480, MCF-7, human lung carcinoma tissue, human tonsil tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

274 kDa

Isotype

IgG

APPLICATION DILUTION

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • WB

  • 1:500-1:1,000

TARGET

UNIPROT #

PROTEIN NAME

IGF2R/M6PR

SYNONYMS

300 kDa mannose 6 phosphate receptor antibody; 300 kDa mannose 6-phosphate receptor antibody; Cation independent mannose 6 phosphate receptor antibody; Cation-independent mannose-6-phosphate receptor antibody; CD222 antibody; CD222 antigen antibody; CI Man 6 P receptor antibody; CI Man-6-P receptor antibody; CI MPR antibody; CI-M6PR antibody; CI-MPR antibody; CIMPR antibody; IGF 2 receptor antibody; IGF 2R antibody; IGF II receptor antibody; IGF-II receptor antibody; IGF2 receptor antibody; Igf2r antibody; Insulin like growth factor 2 receptor antibody; Insulin like growth factor II receptor antibody; Insulin-like growth factor 2 receptor antibody; Insulin-like growth factor II receptor antibody; M6P R antibody; M6P/IGF2 receptor antibody; M6P/IGF2R antibody; M6PR antibody; mannose 6 phosphate receptor antibody; mannose 6 phosphate receptor, cation independent antibody; MPR 300 antibody; MPR300 antibody; MPRI antibody; MPRI_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the MRL1/IGF2R family.

SUBCELLULAR LOCATION

Lysosome membrane, Secreted, Nucleus

FUNCTION

The mannose 6-phosphate/insulin-like growth factor II receptor, IGF-IIR (also designated M6P/IGF2R), is a ubiquitously expressed integral glycoprotein. By binding glycoproteins through two of its extracytoplasmic domains, IGF-IIR mediates the activation of TGF∫1 (a growth inhibitor), the degradation of IGF-II and the transport of lysosomal enzymes. Subsequently, IGF-IIR can form oligomeric complexes, which increase the affinity of IGF-IIR for lysosomal enzymes. Unlike IGF-IR, IGF-IIR does not potentiate the signaling of IGF-I or IGF-II, which have mitogenic, cell survival and insulin-like effects. Therefore, IGF-IIR is characterized as a tumor suppressor. Furthermore, the IGF-IIR gene is located on chromosome 6q26, which is commonly mutated or deleted in several human cancers.