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Human HGF enzyme-linked immunoassay kit (EH0019)

Price: Regular price $499.00

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Catalog# EH0019

Assay type Sandwich ELISA

Standard curve range 10.97~8000 pg/ml

Sensitivity 4.07 pg/ml (50 μl)；6.01 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.02840.02770.0281
10.970.03600.03820.03710.0091
32.920.05210.05010.05110.0231
98.770.11090.09580.10340.0753
296.300.28830.27740.28290.2548
888.890.80030.80330.80180.7738
2666.672.33982.33802.33892.3109
8000.003.96614.04354.00483.9768

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)190.11075.33244.1214.21168.43780.4
Standard Deviation12.473.9219.815.269.0144.5
Coefficient of Variation(%)6.56.96.87.15.93.8

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30360.14-1072.52100609.53

Sensitivity

4.07 pg/ml (50 μl)；6.01 pg/ml (10 μl)

Standard curve range

10.97~8000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human HGF into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 88% to 121% with an overall mean recovery of 108%.

Specifications

Product Type

ELISA Kit

Product Name

Human HGF enzyme-linked immunoassay kit (EH0019)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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