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HRP Conjugated Anti-DYKDDDDK Tag (FLAG) Recombinant Antibody [A2-A4-rFc] - Rabbit IgG (Chimeric)

Usd: 339

Specification

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Catalog# HA601185

HRP Conjugated Anti-DYKDDDDK Tag (FLAG) Recombinant Antibody [A2-A4-rFc] - Rabbit IgG (Chimeric)

Application

  • WB

Reactivity

  • Species independent

Conjugation

  • HRP

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

HRP Conjugated Anti-DYKDDDDK Tag (FLAG) Recombinant Antibody [A2-A4-rFc] - Rabbit IgG (Chimeric)

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Synthetic peptide immune sequence is N-DYKDDDDK-C.

Species Reactivity

Species independent

Validated Applications

WB

Conjugation

HRP

Clone Number

A2-A4-rFc

RRID

AB_3071904

Reactivity Data

WB
Species Independent
Arabidopsis Thaliana
Rice
Tested
N.benthamiana
Tested
Mouse
Tested
Human
Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.05% BSA, 40% Glycerol.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

Target

Function

FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK (where D=aspartic acid, Y=tyrosine, and K=lysine). It is one of the most specific tags and it is an artificial antigen to which specific, high affinity monoclonal antibodies have been developed and hence can be used for protein purification by affinity chromatography and also can be used for locating proteins within living cells. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence, immunoprecipitation or detection by SDS PAGE protein electrophoresis and Western blotting. The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). Additionally, it may be used in tandem, commonly the 3xFLAG peptide: DYKDHD-G-DYKDHD-I-DYKDDDDK (with the final tag encoding an enterokinase cleavage site). It can be fused to the C-terminus or the N-terminus of a protein, or inserted within a protein. The tyrosine residue in the FLAG-tag can be sulfated, which can affect antibody recognition of the FLAG epitope. The FLAG-tag can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag.

Background References

暂无

Synonyms

DDDDK epitope tag antibody

DDDK antibody

ddk antibody

DYKDDDDK antibody

DYKDDDDK epitope tag antibody

DYKDDDDK tag antibody

ECS epitope tag antibody

ECS tag antibody

Enterokinase Cleavage Site epitope tag antibody

Enterokinase Cleavage Site tag antibody

Expand

Images

  • Western blot analysis of DYKDDDDK Tag (FLAG) on different lysates with Rabbit anti-DYKDDDDK Tag (FLAG) antibody (<a href="/products/HA601185" style="font-weight: bold;text-decoration: underline;">HA601185</a>) at different dilutions.<br /><br />Lane 1: N-Flag at 1/1,000 dilution<br />Lane 2: C-Flag at 1/5,000 dilution<br /><br />Lysates/proteins at 50 ng/Lane.<br /><br />Exposure time: 43 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601185" style="font-weight: bold;text-decoration: underline;">HA601185</a>) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours.

    Western blot analysis of DYKDDDDK Tag (FLAG) on different lysates with Rabbit anti-DYKDDDDK Tag (FLAG) antibody (HA601185) at different dilutions.

    Lane 1: N-Flag at 1/1,000 dilution
    Lane 2: C-Flag at 1/5,000 dilution

    Lysates/proteins at 50 ng/Lane.

    Exposure time: 43 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601185) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation