PRODUCT CODE: ER1802-73

Heme Oxygenase 1 (HO-1) Rabbit Polyclonal Antibody (ER1802-73)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

Western blot analysis of Heme Oxygenase 1 (HO-1) on mouse spleen tissue lysate using anti-Heme Oxygenase 1 (HO-1) antibody at 1/500 dilution.
  • Western blot analysis of Heme Oxygenase 1 (HO-1) on mouse spleen tissue lysate using anti-Heme Oxygenase 1 (HO-1) antibody at 1/500 dilution.
  • ICC staining Heme Oxygenase 1 (HO-1) in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Heme Oxygenase 1 (HO-1) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Heme Oxygenase 1 (HO-1) antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Heme Oxygenase 1 (HO-1) antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Heme Oxygenase 1 (HO-1) antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Heme Oxygenase 1 (HO-1) antibody. Counter stained with hematoxylin.
Western blot analysis of Heme Oxygenase 1 (HO-1) on mouse spleen tissue lysate using anti-Heme Oxygenase 1 (HO-1) antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Heme Oxygenase 1 (HO-1) Rabbit Polyclonal Antibody (ER1802-73)

Immunogen

Synthetic peptide of n-terminal human heme oxygenase 1 (ho-1).

Host

Rabbit

Positive Control

Mouse spleen tissue lysate, 293T, HepG2, human spleen tissue, human placenta tissue, human small intestine tissue, mouse small intestine tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

33 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:500

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Heme Oxygenase 1 (HO-1)

SYNONYMS

32 kD antibody; bK286B10 antibody; D8Wsu38e antibody; heat shock protein 32 kD antibody; heat shock protein 32kD antibody; Heat shock protein antibody; Heme oxygenase (decycling) 1 antibody; Heme oxygenase 1 antibody; Hemox antibody; HMOX 1 antibody; Hmox antibody; Hmox1 antibody; HMOX1_HUMAN antibody; HO 1 antibody; HO antibody; HO-1 antibody; HO1 antibody; Hsp32 antibody

SEQUENCE SIMILARITIES

Belongs to the heme oxygenase family.

TISSUE SPECIFICITY

Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level).

SUBCELLULAR LOCATION

Endoplasmic reticulum.

FUNCTION

Heme oxygenases are microsomal enzymes that cleave heme to produce the antioxidant biliverdin, inorganic iron and carbon monoxide (CO). The activity of Heme Oxygenase 1 (HO-1), also designated HSP 32, is highly inducible in response to numerous stimuli, including heme, heavy metals, hormones and oxidative stress. Heme Oxygenase 2, in contrast, appears to be constituitively expressed in mammalian tissues. Heme Oxygenase 2 is involved in the production of carbon monoxide (CO) in brain, where CO is thought to act as a neurotransmitter. The CO signaling system closely parallels the signaling pathway involving nitric oxide, and regulation of the two systems is closely linked. Heme Oxygenase 3 is found in the spleen, liver, thymus, prostate, heart, kidney, brain and testis. A poor heme catalyst, Heme Oxygenase 3 has two heme regulatory motifs that may be involved in heme binding.

CITATIONS

  • Wang, J., Bai, Y., Yin, S.,...

    Circadian clock gene BMAL1 reduces urinary calcium oxalate stones formation by regulating NRF2/HO-1 pathway. Life sciences, 265, 118853.