Heme Oxygenase 1 (HO-1) Recombinant Rabbit Monoclonal Antibody [PSH02-76] (HA721854)
Catalog# HA721854
Rabbit Monoclonal to Heme Oxygenase 1(HO-1)
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
Western blot analysis of Heme Oxygenase 1 (HO-1) on different lysates with Rabbit anti-Heme Oxygenase 1 (HO-1) antibody (HA721854) at 1/5,000 dilution.
Lane 1: A549-si NT cell lysate
Lane 2: A549-si Heme Oxygenase 1 (HO-1) cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 9 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721854) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Heme Oxygenase 1 (HO-1) Recombinant Rabbit Monoclonal Antibody [PSH02-76] (HA721854)
Immunogen
Synthetic peptide within human heme oxygenase 1 aa 1-50 / 288.
Host
Rabbit
Positive Control
HeLa cell lysate, A549 cell lysate, RAW264.7 cell lysate, A549, RAW264.7, human renal cell carcinoma tissue, mouse spleen tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PSH02-76
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:5,000
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IF-Cell
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1:100
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IHC-P
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 33 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Heme Oxygenase 1(HO-1)
SYNONYMS
32 kD antibody;bK286B10 antibody;D8Wsu38e antibody;heat shock protein 32 kD antibody;heat shock protein 32kD antibody;Heat shock protein antibody;Heme oxygenase (decycling) 1 antibody;Heme oxygenase 1 antibody;Hemox antibody;HMOX 1 antibody;Hmox antibody;Hmox1 antibody;HMOX1_HUMAN antibody;HO 1 antibody;HO antibody;HO-1 antibody;HO1 antibody;Hsp32 antibody
SUBCELLULAR LOCATION
Endoplasmic reticulum membrane.
FUNCTION
Heme oxygenases are microsomal enzymes that cleave heme to produce the antioxidant biliverdin, inorganic iron and carbon monoxide (CO). The activity of Heme Oxygenase 1 (HO-1), also designated HSP 32, is highly inducible in response to numerous stimuli, including heme, heavy metals, hormones and oxidative stress. Heme Oxygenase 2, in contrast, appears to be constituitively expressed in mammalian tissues. Heme Oxygenase 2 is involved in the production of carbon monoxide (CO) in brain, where CO is thought to act as a neurotransmitter. The CO signaling system closely parallels the signaling pathway involving nitric oxide, and regulation of the two systems is closely linked. Heme Oxygenase 3 is found in the spleen, liver, thymus, prostate, heart, kidney, brain and testis. A poor heme catalyst, Heme Oxygenase 3 has two heme regulatory motifs that may be involved in heme binding.
CITATIONS
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Jianjun Chang,et al
Protective role of aconitate decarboxylase 1 in neuroinflammation-induced dysfunctions of the paraventricular thalamus and sleepiness
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WB
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Mouse
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Citation
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Zhang Xiaomin,et al
Boric Acid Alleviates Lipopolysaccharide-Induced Acute Lung Injury in Mice
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WB
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Mouse
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Citation
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Wu Peiyao,et al
Periodontal pathogen Fusobacterium nucleatum infection accelerates hepatic steatosis in high‐fat diet‐fed ApoE knockout mice by inhibiting Nrf2/Keap1 signaling
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WB
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Mouse
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Citation
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