GSC Recombinant Rabbit Monoclonal Antibody [JE51-35]
Catalog# HA722460
GSC Recombinant Rabbit Monoclonal Antibody [JE51-35]
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WB
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Human
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Rat
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Green monkey
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unconjugated
Overview
Product Name
GSC Recombinant Rabbit Monoclonal Antibody [JE51-35]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human GSC aa 1-50 / 257.
Species Reactivity
Human, Rat, Green monkey
Validated Applications
WB
Molecular Weight
Predicted band size: 28 kDa
Positive Control
MCF7 cell lysate, HepG2 cell lysate, BxPC-3 cell lysate, HeLa cell lysate, COS-1 cell lysate.
Conjugation
unconjugated
Clone Number
JE51-35
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Regulates chordin (CHRD). May play a role in spatial programing within discrete embryonic fields or lineage compartments during organogenesis. In concert with NKX3-2, plays a role in defining the structural components of the middle ear; required for the development of the entire tympanic ring. Probably involved in the regulatory networks that define neural crest cell fate specification and determine mesoderm cell lineages in mammals. This gene encodes a member of the bicoid subfamily of the paired (PRD) homeobox family of proteins. The encoded protein acts as a transcription factor and may be autoregulatory. A similar protein in mice plays a role in craniofacial and rib cage development during embryogenesis.
Background References
1. Parry D.A., Logan C.V., Stegmann A.P., Abdelhamed Z.A., Calder A., Khan S., Bonthron D.T., Clowes V., Sheridan E., Johnson C.A. SAMS, a syndrome of short stature, auditory-canal atresia, mandibular hypoplasia, and skeletal abnormalities is a unique neurocristopathy caused by mutations in Goosecoid. Am. J. Hum. Genet. 93:1135-1142 (2013)
Subcellular Location
Nucleus.
Synonyms
Goosecoid antibody
goosecoid homeobox antibody
GSC antibody
GSC_HUMAN antibody
Homeobox protein goosecoid antibody
Images
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Western blot analysis of GSC on different lysates with Rabbit anti-GSC antibody (HA722460) at 1/1,000 dilution.
Lane 1: MCF7 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: BxPC-3 cell lysate
Lane 4: HeLa cell lysate
Lane 5: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 46 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722460) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"