PRODUCT CODE: EM1701-23

Grp75 Mouse Monoclonal Antibody [6-A9] (EM1701-23)

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Grp75 on different cell lysates using anti-Grp75 antibody at 1/1,000 dilution.<br />
  Positive control:<br />
 Lane 1: NIH-3T3<br />
         Lane 2: HepG2<br />
   Lane 3: K562 <br />
           Lane 4: L929
  • Western blot analysis of Grp75 on different cell lysates using anti-Grp75 antibody at 1/1,000 dilution.<br />
  Positive control:<br />
 Lane 1: NIH-3T3<br />
         Lane 2: HepG2<br />
   Lane 3: K562 <br />
           Lane 4: L929
  • ICC staining Grp75 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Grp75 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Grp75 (green) in SK-Br-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Grp75 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Grp75 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse oviduct tissue using anti-Grp75 antibody. Counter stained with hematoxylin.
Western blot analysis of Grp75 on different cell lysates using anti-Grp75 antibody at 1/1,000 dilution.
Positive control:
Lane 1: NIH-3T3
Lane 2: HepG2
Lane 3: K562
Lane 4: L929

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Grp75 Mouse Monoclonal Antibody [6-A9] (EM1701-23)

Immunogen

Peptide

Host

Mouse

Positive Control

HepG2, L929, K562, NIH-3T3, A549, SK-Br-3, rat epididymis tissue, mouse liver tissue, mouse oviduct tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

6-A9

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

74 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Grp75

SYNONYMS

75 kDa glucose regulated protein antibody; 75 kDa glucose-regulated protein antibody; CSA antibody; Glucose Regulated Protein antibody; Grp 75 antibody; GRP-75 antibody; GRP75 antibody; GRP75_HUMAN antibody; Heat shock 70 kDa protein 9 antibody; Heat shock 70kD protein 9 antibody; heat shock 70kDa protein 9 antibody; Heat shock 70kDa protein 9B antibody; Heat shock protein 74 kDa A antibody; Heat shock protein A antibody; Heat shock protein cognate 74 antibody; Hsc74 antibody; Hsp74 antibody; Hsp74a antibody; HSPA9 antibody; Hspa9a antibody; HSPA9B antibody; MGC4500 antibody; mitochondrial antibody; Mortalin 2 antibody; Mortalin antibody; Mortalin perinuclear antibody; Mortalin2 antibody; MOT 2 antibody; MOT antibody; MOT2 antibody; Mthsp70 antibody; p66 mortalin antibody; P66 MOT antibody; PBP74 antibody; Peptide binding protein 74 antibody; Peptide-binding protein 74 antibody; Stress 70 protein mitochondrial antibody; Stress 70 protein mitochondrial precursor antibody; Stress-70 protein antibody

SEQUENCE SIMILARITIES

Belongs to the heat shock protein 70 family.

SUBCELLULAR LOCATION

Mitochondrion. Nucleus.

FUNCTION

The HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. HSC 70, GRP 75 and GRP 78 are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. GRP 75 and GRP 78 are unresponsive to heat stress and are induced by glucose deprivation. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP 75 and GRP 78 are unresponsive to heat stress and are induced by glucose deprivation.

CITATIONS

  • Xu, Xiu-Ping et al.

    Ang II-AT2R increases mesenchymal stem cell migration by signaling through the FAK and RhoA/Cdc42 pathways in vitro. | Stem Cell Research & Therapy [2017]