GPX4 Rabbit Polyclonal Antibody (ER1803-15)
Catalog# ER1803-15
Rabbit Polyclonal to GPX4
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
Lane 1: Rat testis tissue lysate (20 µg/Lane)
Lane 2: Mouse testis tissue lysate (20 µg/Lane)
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 2 minutes;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-15) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
GPX4 Rabbit Polyclonal Antibody (ER1803-15)
Immunogen
Recombinant protein within human gpx4 aa 1-100 / 197.
Host
Rabbit
Positive Control
Rat testis tissue lysate, HepG2, LOVO, MCF-7, mouse testis tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:500-1:1,000
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IF-Cell
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1:50-1:100
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IHC-P
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1:50 -1:600
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FC
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 22 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
GPX4
SYNONYMS
Glutathione peroxidase 4 antibody;GPX 4 antibody;GPX-4 antibody;GPX4 antibody;GPX4_HUMAN antibody;GSHPx-4 antibody;MCSP antibody;mitochondrial antibody;PHGPx antibody;Phospholipid hydroperoxidase antibody;Phospholipid hydroperoxide glutathione peroxidase antibody;Phospholipid hydroperoxide glutathione peroxidase mitochondrial antibody;snGPx antibody;snPHGPx antibody;Sperm nucleus glutathione peroxidase antibody
SEQUENCE SIMILARITIES
Belongs to the glutathione peroxidase family.
TISSUE SPECIFICITY
Present primarily in testis. Expressed in platelets (at protein level).
SUBCELLULAR LOCATION
Cytoplasm. Mitochondrion.
FUNCTION
Essential antioxidant peroxidase that directly reduces phospholipid hydroperoxide even if they are incorporated in membranes and lipoproteins . Can also reduce fatty acid hydroperoxide, cholesterol hydroperoxide and thymine hydroperoxide . Plays a key role in protecting cells from oxidative damage by preventing membrane lipid peroxidation. Required to prevent cells from ferroptosis, a non-apoptotic cell death resulting from an iron-dependent accumulation of lipid reactive oxygen species . The presence of selenocysteine (Sec) versus Cys at the active site is essential for life: it provides resistance to overoxidation and prevents cells against ferroptosis . The presence of Sec at the active site is also essential for the survival of a specific type of parvalbumin-positive interneurons, thereby preventing against fatal epileptic seizures . May be required to protect cells from the toxicity of ingested lipid hydroperoxides . Required for normal sperm development and male fertility. Essential for maturation and survival of photoreceptor cells. Plays a role in a primary T-cell response to viral and parasitic infection by protecting T-cells from ferroptosis and by supporting T-cell expansion.
CITATIONS
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Zhao Jingyuan,et al
Heme oxygenase-1 is involved in the erastin-induced ferroptosis in acute myeloid leukemia cells
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WB
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Human
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Citation
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H1N1 influenza virus infection through NRF2-KEAP1-GCLC pathway induces ferroptosis in nasal mucosal epithelial cells
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WB
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Mouse
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Citation
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Tian, C., Zhao, J., Xiong, Q., Yu, H., & Du, H.
Secondary iron overload induces chronic pancreatitis and ferroptosis of acinar cells in mice
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WB
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Mouse
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Citation
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Xia Yu,et al
Artesunate-loaded solid lipid nanoparticles resist esophageal squamous cell carcinoma by inducing Ferroptosis through inhibiting the AKT/mTOR signaling
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WB
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Mouse
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Citation
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Qilu Fang,et al
CAPE activates AMPK and Foxo3 signaling to induce growth inhibition and ferroptosis in triple-negative breast cancer
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WB
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Human
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Citation
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Hydroxysafflor yellow A and anhydrosafflor yellow B alleviate ferroptosis and parthanatos in PC12ácells injured by OGD/R
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WB
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Rat
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Citation
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Li Xuehui,et al
Biosynthetic deficiency of docosahexaenoic acid causes nonalcoholic fatty liver disease and ferroptosis-mediated hepatocyte injury
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WB
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Zebrafish
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Citation
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-
Artemisitene Alters LPS-Induced Oxidative stress, inflammation and Ferroptosis in Liver Through Nrf2/HO-1 and NF-kB Pathway
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WB
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Mouse
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Citation
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Yongjing Zhang,et al
Corydalis yanhusuo extract and its pharmacological substances alleviate food allergy by inhibiting mast cells activation via PLC/PKC/STAT3 pathway
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WB
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Rat
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Citation
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Chen, Y. M., Xu, W., Liu, Y., Zhang, J. H., Yang, Y. Y., Wang, Z. W., Sun, D. J., Li, H., Liu, B., & Chen, L. X.
Anomanolide C suppresses tumor progression and metastasis by ubiquitinating GPX4-driven autophagy-dependent ferroptosis in triple negative breast cancer
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WB
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IP
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Mouse
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Citation
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Wang H, Guo S, Wang B, et al
Carnosine attenuates renal ischemia-reperfusion injury by inhibiting GPX4-mediated ferroptosis
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WB
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Mouse
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Citation
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Li Yong,et al
A DNA tetrahedron-based ferroptosis-suppressing nanoparticle: superior delivery of curcumin and alleviation of diabetic osteoporosis
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IF
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Mouse
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Citation
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Zhao Jing,et al
Differentiation of intestinal stem cells toward goblet cells under systemic iron overload stress are associated with inhibition of Notch signaling pathway and ferroptosis
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WB
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Mouse
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Citation
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