Glucose Transporter GLUT1 Recombinant Rabbit Monoclonal Antibody [SA0377] (ET1601-10)
Catalog# ET1601-10
Rabbit Monoclonal to Glucose Transporter GLUT1
-
WB
-
IF-Cell
-
IF-Tissue
-
IHC-P
-
FC
-
Human
-
Mouse
-
Rat
Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Glucose Transporter GLUT1 antibody (ET1601-10) at 1/500 dilution and competitor's antibody at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Glucose Transporter GLUT1 Recombinant Rabbit Monoclonal Antibody [SA0377] (ET1601-10)
Immunogen
Synthetic peptide within human glut1 aa 443-492 / 492.
Host
Rabbit
Positive Control
HeLa cell lysate, PC-12 cell lysate, HeLa, HT-29 cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, L-929 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, Jurkat, NIH/3T3, C6, human liver tissue, human placenta tissue, human liver carcinoma tissue, human kidney tissue, mouse liver tissue, mouse kidney tissue, HepG2, human lung cancer tissue, human liver tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SA0377
RRID
APPLICATION DILUTION
-
WB
-
1:5,000-1:50,000
-
IF-Cell
-
1:500
-
IF-Tissue
-
1:50-1:200
-
IHC-P
-
1:50-1:200
-
FC
-
1:500-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 54 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Glucose Transporter GLUT1
SYNONYMS
Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) antibody;CSE antibody;DYT17 antibody;DYT18 antibody;DYT9 antibody;EIG12 antibody;erythrocyte/brain antibody;Erythrocyte/hepatoma glucose transporter antibody;facilitated glucose transporter member 1 antibody;Glucose transporter 1 antibody;Glucose transporter type 1 antibody;Glucose transporter type 1, erythrocyte/brain antibody;GLUT antibody;GLUT-1 antibody;GLUT1 antibody;GLUT1DS antibody;GLUTB antibody;GT1 antibody;GTG1 antibody;Gtg3 antibody;GTR1_HUMAN antibody;HepG2 glucose transporter antibody;HTLVR antibody;Human T cell leukemia virus (I and II) receptor antibody;MGC141895 antibody;MGC141896 antibody;PED antibody;RATGTG1 antibody;Receptor for HTLV 1 and HTLV 2 antibody;SLC2A1 antibody;Solute carrier family 2 (facilitated glucose transporter), member 1 antibody;Solute carrier family 2 antibody;Solute carrier family 2, facilitated glucose transporter member 1 antibody
SEQUENCE SIMILARITIES
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
TISSUE SPECIFICITY
Detected in erythrocytes (at protein level). Expressed at variable levels in many human tissues.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation at Ser-226 by PKC promotes glucose uptake by increasing cell membrane localization.
SUBCELLULAR LOCATION
Cell membrane, Melanosome
FUNCTION
Glucose transporter 1 (or GLUT1), also known as solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1), is a uniporter protein that in humans is encoded by the SLC2A1 gene. GLUT1 facilitates the transport of glucose across the plasma membranes of mammalian cells. This gene encodes a major glucose transporter in the mammalian blood-brain barrier. The encoded protein is found primarily in the cell membrane and on the cell surface, where it can also function as a receptor for human T-cell leukemia virus (HTLV) I and II. One good source of GLUT1 is erythrocyte membranes. GLUT1 accounts for 2 percent of the protein in the plasma membrane of erythrocytes. GLUT1, found in the plasma membrane of erythrocytes, is a classic example of a uniporter. After glucose is transported into the erythrocyte, it is rapidly phosphorylated, forming glucose-6-phosphate, which cannot leave the cell. Mutations in this gene can cause GLUT1 deficiency syndrome 1, GLUT1 deficiency syndrome 2, idiopathic generalized epilepsy 12, dystonia 9, and stomatin-deficient cryohydrocytosis.
CITATIONS
-
Liu, Y., Chen, Y., Wang, Y., Jiang, S., Lin, W., Wu, Y., Li, Q., Guo, Y., Liu, W., & Yuan, Q.
DNA demethylase ALKBH1 promotes adipogenic differentiation via regulation of HIF-1 signaling
-
WB
-
Human
-
Citation
-
-
Gao, F., Liang, T., Lu, Y. W., Fu, X., Dong, X., Pu, L., Hong, T., Zhou, Y., Zhang, Y., Liu, N., Zhang, F., Liu, J., Malizia, A. P., Yu, H., Zhu, W., Cowan, D. B., Chen, H., Hu, X., Mably, J. D., Wang, J., … Chen, J.
A defect in mitochondrial protein translation influences mitonuclear communication in the heart
-
WB
-
Mouse
-
Citation
-
-
Yang, F., Zhou, L., Shen, Y., Zhao, S., Zheng, Y., Men, R., Fan, X., & Yang, L.
Metabolic heterogeneity caused by HLA-DRB1*04:05 and protective effect of inosine on autoimmune hepatitis
-
WB
-
Citation
-
Popup
Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.
Age verification
By clicking enter you are verifying that you are old enough to consume alcohol.