GADD45B Recombinant Rabbit Monoclonal Antibody [PSH09-52]
Catalog# HA723114
GADD45B Recombinant Rabbit Monoclonal Antibody [PSH09-52]
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WB
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Human
-
Mouse
-
Rat
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HA751299
不含抗保成分
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unconjugated
Overview
Product Name
GADD45B Recombinant Rabbit Monoclonal Antibody [PSH09-52]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human GADD45B aa 1-160.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 18 kDa
Positive Control
HeLa cell lysate, Huh7 cell lysate, MCF7 cell lysate, A431 cell lysate, PC-12 cell lysate, Mouse testis tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
PSH09-52
Reactivity Data
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:2,000
Target
Function
Growth arrest and DNA-damage-inducible, beta, also known as GADD45B, is a protein which in humans is encoded by the GADD45B gene. This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The genes in this group respond to environmental stresses by mediating activation of the p38/JNK pathway. This activation is mediated via their proteins binding and activating MTK1/MEKK4 kinase, which is an upstream activator of both p38 and JNK MAPKs. The function of these genes or their protein products is involved in the regulation of growth and apoptosis. These genes are regulated by different mechanisms, but they are often coordinately expressed and can function cooperatively in inhibiting cell growth. Gadd45b is required for activity-induced DNA demethylation of specific promoters and expression of corresponding genes necessary for adult neurogenesis, including brain-derived neurotrophic factor and fibroblast growth factor. Hence GADD45B is implicated in affecting synaptic plasticity.
Background References
1. Gong L et al. GADD45B Facilitates Metastasis of Ovarian Cancer Through Epithelial-Mesenchymal Transition. Onco Targets Ther. 2021 Jan
2. Xie M et al. GADD45A and GADD45B as Novel Biomarkers Associated with Chromatin Regulators in Renal Ischemia-Reperfusion Injury. Int J Mol Sci. 2023 Jul
Subcellular Location
Cytoplasm, nucleus.
Synonyms
GA45B_HUMAN antibody
GADD45 Beta antibody
Gadd45b antibody
GADD45BETA antibody
Growth arrest and DNA damage inducible 45 beta antibody
Growth arrest and DNA damage inducible beta antibody
Growth arrest and DNA damage-inducible gene GADD45, beta antibody
Growth arrest and DNA damage-inducible protein GADD45 beta antibody
Growth arrest and DNA-damage-inducible protein GADD45 beta antibody
MY118 antibody
ExpandImages
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Western blot analysis of GADD45B on different lysates with Rabbit anti-GADD45B antibody (HA723114) at 1/2,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Huh7 cell lysate (20 µg/Lane)
Lane 3: MCF7 cell lysate (20 µg/Lane)
Lane 4: A431 cell lysate (20 µg/Lane)
Lane 5: PC-12 cell lysate (20 µg/Lane)
Lane 6: Mouse testis tissue lysate (40 µg/Lane)
Lane 7: Mouse brain tissue lysate (40 µg/Lane)
Lane 8: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 18 kDa
Observed band size: 18 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723114) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
GADD45B Recombinant Rabbit Monoclonal Antibody [PSH09-52] - BSA and Azide free
Application: WB
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
