Applications
-
WB
-
ELISA
REACTIVITY
-
Avian avulavirus 1
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
Fusion glycoprotein F0 Rabbit Polyclonal Antibody (ER1803-51)
Immunogen
Synthetic peptide corresponding to avian avulavirus 1 fusion glycoprotein f0 aa 540-554.
Host
Rabbit
Positive Control
Fusion glycoprotein F0 transfected HEK293 cell.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Peptide affinity purified.
MOLECULAR WEIGHT
59 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1,000
-
ELISA
-
1:5,000-1:10,000
TARGET
UNIPROT #
PROTEIN NAME
Fusion glycoprotein F0
SYNONYMS
Fusion glycoprotein F0 antibody; Fusion glycoprotein F1 antibody; Fusion glycoprotein F2 antibody; Fusion protein (F) antibody; RSV fusion protein antibody
SEQUENCE SIMILARITIES
Belongs to the paramyxoviruses fusion glycoprotein family.
SUBCELLULAR LOCATION
SwissProt: W8CLH7 (Avian avulavirus 1)
FUNCTION
During virus entry, induces fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm. The fusogenic activity is inactive untill entry into host cell endosome, where a furin-like protease cleaves off a small peptide between F1 and F2. Interacts directly with heparan sulfate and may participate in virus attachment. Furthermore, the F2 subunit was identifed as the major determinant of RSV host cell specificity. Later in infection, proteins F expressed at the plasma membrane of infected cells can mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis. The fusion protein is also able to trigger p53-dependent apoptosis.